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Lab 1.2 Plasmid DNA characterization Biochem > Lecture > Flashcards

Lecture Flashcards

1
Q

Band mobility

A

How fast bands travel
Separate dna and rna by size
Small fragments migrate faster

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2
Q

Band speed and concentration

A

Heavy band resolve in low gel concentration
Light band resolve in high gel concentration

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3
Q

Resolution

A

How many pixel it can distinguish from each other

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4
Q

Processes done

A

Quantification of intact dna via electrophoresis
Detection of contaminants via spectrophotometry

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5
Q

Intense band

A

Indicate more DNA

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6
Q

Abs 230

A

Protein backbones and salts

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7
Q

Abs 260

A

Nucleotides/DNA

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8
Q

Abs 280

A

Proteins/ aromatic amino acid

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9
Q

Phenol

A

Absorbed in all absorbance but peak at 270

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10
Q

Starch

A

Particulate contaminant but absorbance across the board

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11
Q

Salt contamination

A

Huge absorbance at 230

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12
Q

Protein contamination

A

Huge absorbance at 230 but larger than salt and will move peak a bit bc 270 is max absorbance

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13
Q

Phenol contamination

A

Peak will move and be higher

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14
Q

Starch contamination

A

Higher peak

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15
Q

Contamination and values

A

Salt and protein will decrease spectrophotmetry value

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16
Q

Abs 260/280

A

Wont respond to salt contamination
Will respond to protein and phenol

17
Q

X axis michaelis menten

A

Substrate concentration

18
Q

Y axis michaelis menten

A

Velocity/ rate of reaction

19
Q

First order kinetics

A

Reaction rate proportional to substrate concentration
Curve and plateau at vmax

20
Q

Zero order

A

Straight line

21
Q

Restriction enzyme

A

Endonuclease cut within DNA

22
Q

Observe circular to linear change

A

Mobility in gel will change

23
Q

Supercoiled

A

More packed allowing it to pass through faster

24
Q

Open circular

A

Can dimerize making it harder to travel

Lab 1.2 Plasmid DNA characterization Biochem (5 decks)

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