LESSON #2: ENZYME KINEMATICS

Question 1

A double reciprocal plot of michaelis menten constant which yield a straight line

Answer 1

Lineweaver burk plot

Question 2

Are those who proceed at a rate exactly proportional to the concentration of one reactant

Answer 2

First-order reaction

Question 3

Are those in which rate is proportional to the product of the concentration of two reactants or square of the concentration of one reactant

Answer 3

Second-order reaction

Question 4

A higher concentration of substrate will no longer result in increased reaction rate

Answer 4

Zero-order reaction

Question 5

Factors that influence enzymatic reaction

Answer 5

1. Substrate concentration
2. Enzyme concentration
3. pH
4. Temperature
5. Cofactors
6. Activators
7. Inhibitors

Question 6

The higher the enzyme level, the faster the reaction will proceed.

Answer 6

Enzyme concentration

Question 7

-pH most enzymes
-Changes in __ may denature the enzyme
-Each enzyme operates within specific pH range

Answer 7

7.0-8.0

Question 8

Temperature
- _____ degrees celsius
- denaturation at ________ degrees celsius
- Assay temperature: ___, ____, _____

Answer 8

Temperature
- 37 degrees celsius
- denaturation at 40-50 degrees celsius
- Assay temperature: 25, 30, 37

Question 9

nonprotein entities that must bind to particular enzymes before a reaction occurs
Activators: _____ or ______
Coenzymes: _______, _______, _______

Answer 9

Cofactors
metallic or nonmetallic
nucleotide, phosphate and vitamins

Question 10

-proper substrate binding
- link substrate to the enzyme or coenzyme
-undergo oxidation or reduction

Answer 10

Activators

Question 11

Interfere with enzyme reactions

Answer 11

inhibitors

Question 12

What are the three inhibitor

Answer 12

• competitive
  -noncompetitive
  -uncompetitive

Question 13

MEASUREMENT OF ENZYME ACTIVITY
Increase in ______ concentration
Decrease in ______ concentration
Decrease or increase in _________ concentration
An increase in the concentration of the altered coenzyme

Answer 13

product
substrate
coenzyme

Question 14

What are the three enzymatic assays

Answer 14

Coupled enzyme assay
Fixed time method
Continuous monitoring method

Question 15

-Coupling the activity of the enzyme being tested to another
-more easily detectable, enzyme
-indirect measurement

Answer 15

Coupled enzyme assay

Question 16

• reactants are combined
• reaction proceeds for a designated time
• reaction is stopped
• measurement is made of the amount of reaction is occured

Answer 16

Fixed time method (End point_

Question 17

Amount of enzyme that will catalyze the reaction of 1 micromole of substrate per minute under specified conditions

Answer 17

International Unit

Question 18

Enzyme concentration

Answer 18

International Unit

Question 19

Amount of enzyme that will catalyze the reaction of 1 mole of substrate per second under specified conditions

Answer 19

Katal Unit