LESSON #4: AST, ALT, ALP, ACP Flashcards
ASPARTATE AMINOTRANSFERASE
Systemic name:
Function:
Product:
Former name:
Cofactor:
Systemic name: L aspartate 2 oxoglutarate aminotransferase
Function: Transfer of an amino group between aspartate and alpha keto acids to form oxaloacetate and glutamate
Product: oxaloacetate and glutamate
Serum glutamic oxaloacetic transaminase (SGOT or GOT)
Cofactor: Pyridoxal Phosphate (Vitamin B 6)
2 Oxoglutarate - _________
L aspartate - ___________
L glutamate
Oxaloacetate
ALANINE AMINOTRANSFERASE
Systemic name:
Function:
Product:
Former name:
Cofactor:
Systemic name: L alanine: 2 oxaloglutarate aminotransferase
Function: Transfer of an amino group between aspartate and alpha keto acids to form glutamate and pyruvatre
Product: glutamate and pyruvate
Former name: Serum glutamic pyruvic transaminase
Cofactor: Pyridoxal phosphate ( vitamin B6)
AST intracellular level _____ times than plasma
7000
ALT intracellular level _____ times than plasma
3000
In isoenzymes AST occurs on tow forms
Cytoplasmic AST
Mitochondrial AST
predominant form in serum
cell cytoplasm
increased in disorders producing cellular necrosis
Mitochondria
2 AST highest tissue sources
Cardiac tissue - highest
Liver - second highest
ALT highest tissue source
Liver
Diseases that damage the hepatocyte directly
Hepatocellular (ALT and AST)
example of hepatobiliary
gallstone or obstruction of the bile duct
ALT: ___________
Cytoplasmic AST: __________
Mitochondrial AST: __________
ALT:47 hours
Cytoplasmic AST: 17 hours
Mitochondrial AST: half-life of 87 hours
Catalyze the hydrolysis of various phosphomonoesters at a certain pH
Phosphatases
Not tested anymore in laboratories
Acid Phosphatases
Four major isoenzyme
Placental ALP
Liver ALP
Bone ALP
Intestinal ALP
Isoenzyme that not use when someone is pregnant
Placental ALP
ALP is used to differentiate ________, _______, ________
prehepatic, posthepatic, hepatic jaundice
Lab only measures _______
ALP
Fast isoenzyme
LiBoPlain
