LESSON 8a

Question 1

Ferment glucose, reduce nitrate to nitrite

Answer 1

Enterobacteriaceae

Question 2

Enteric bacteria which tolerate bile salts in _____

Answer 2

MacConkey agar

Question 3

Cause a variety of clinical infections

Answer 3

Enterobacteriaceae

Question 4

Enterobacteriaceae
Major enteric and systemic pathogens

Answer 4

Escherichia coli
Salmonella
serotypes
Yersinia species

Question 5

Non-pathogenic enterobacteriaceae:

Answer 5

Hafnia sp. and Erwinia sp

Question 6

formerly only used to describe enterobacteria capable of
fermenting lactose, is now sometimes used to describe other members of the
family.

Answer 6

coliform

Question 7

Differentiation of Enterobacteriaceae

Serotyping of E. coli, Salmonella and Yersinia species:
 ______ with antisera are used to detect _______ and ______ antigens in all three species and sometimes
detection of _______ antigens is carried out (Fig. 3). Serotyping
allows identification of the organisms involved in disease outbreaks
and has applications in epidemiological investigations.

Answer 7

*Slide agglutination tests
*O (somatic)
and H (flagellar)
K (capsular)

Question 8

 Slide agglutination tests with antisera are used to detect O (somatic)
and H (flagellar) antigens in all three species and sometimes
detection of K (capsular) antigens is carried out (Fig. 3). Serotyping
allows identification of the organisms involved in disease outbreaks
and has applications in epidemiological investigations.

Answer 8

Serotyping of E. coli
Salmonella
Yersinia species

Question 9

Molecular techniques, usually based on PCR, are increasingly used both for
detection of enterobacteria in _______

Answer 9

clinical or food samples and for identification of
suspect isolates.