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2023 SEM 1 ANAPHY (LEC) > microscope > Flashcards

microscope Flashcards

1
Q

what occurs on the 1st century

A

the Romans experimented with glass.
they found the objects to appear bigger

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2
Q

in the 12th century, Salvino D’Armate made the

A

first eye glass

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3
Q

when and who did the 2 dutch made the first compound microscope. how did they do it

A

1590
zacharias jansen and his dad
by mounting 2 lenses in a tab

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4
Q

who developed the compound microscope with convex and concave mirror

A

galileo galilei

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5
Q

who is the author of the book micrograph

A

Robert Hooke

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6
Q

micrograph is a book that

A

documented wide range of observations through microscope

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7
Q

who achieve greater magnification of the microscope and when

A

anton van leeuwenheok
1674

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8
Q

in 1826, what did joseph jackson acheve

A

achromatic lense to eradicate the chromatic effect

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9
Q

Ernst Ruska build the…. in…

A

first electron microscope
1931

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10
Q

what is the oldest ad simplest MC

A

optical microscope

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11
Q

single lens in MC is called

A

loupe

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12
Q

what is the source of light used in simple MC

A

natural light

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13
Q

is there condenser in simple MC

A

no

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14
Q

is the magnification adjustable in simple MC

A

no

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15
Q

what is the most commonly used MC

A

compound MC

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16
Q

compound MC is also known as

A

biological microscope

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17
Q

do compound microscope uses condenser

A

yes

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18
Q

what is being used to magnify image in compound microscope

A

compound lens system

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19
Q

dissecting microscope is also known as

A

stereoscopic MC

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20
Q

stereoscopic MC is used to …

A

manipulate
probe
separate tissues

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21
Q

why do dissecting MC has an image of 3D

A

to observe live specimen
to perform dissectionw

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22
Q

what is the source of light for electron MC

A

electron

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23
Q

what MC is used in biomedical research

A

electron MC

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24
Q

what are the 2 types of electron MC

A

scanning electron MC
transmission electron MC

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25
Q

what are the similarities between TEM and SEM

A

they both produce a high magnified image

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26
Q

what are the difference between TEM and SEM

A

TEM - 20 000x
SEM - 20 millionx
total magnification differs

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27
Q

which MC appears brightly lit against dark backgrounds

A

dark field

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28
Q

which MC is usually used in platelet

A

phase contrast MC

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29
Q

name the part that supports MC

A

base

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30
Q

name the part that supports tube and connects to the base

A

arm

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31
Q

name the part that holds 2 or more objective lens and can rotate easily to change power

A

revolving nose system

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32
Q

name the part that has a horizontal platform with central opening

A

stage

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33
Q

name the part that gives controlled movement to the objects on the slides

A

mechanical stage

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34
Q

name the part that reflects light rays from source to object. one side is concave mirror while the other is a plane mirror

A

mirror

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35
Q

name the part that focuses the light

A

condenser

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36
Q

name the part that regulate the amount of light intensity passing into the condenser

A

diaphragm

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37
Q

name the part that on and off the light

A

power switch

38
Q

name the part that holds the slides

A

stage clips

39
Q

name the part that has a maximum and minimum of lowering stage

A

stage stop

40
Q

name the part that moves stage left and right

A

stage adjustment

41
Q

name the part that focus light of specimens and control the light elimination

A

condenser adjustment

42
Q

name the part that reguates light intensity

A

rheostat control knob

43
Q

name the part that raises or lowers the stage to bring image into focus

A

coarse adjustment

44
Q

name the part that brings image to sharp focus

A

fine adjustment

45
Q

what are the type of magnifying object

A

4x scanner objective
10x low power objective
40x high power objective
100x oil immersion objective

46
Q

how to find the total magnification

A

lens of your eye piece x objective used

47
Q

what is the power of eyepiece

A

10x or 15x

47
Q

the eyepiece consists of…

A

upper eye lens and
lower field lens

48
Q

what is the first vision instrument

A

reading stone

49
Q

what AD is reading stone invented

A

1000AD

50
Q

what is the optical instrument designed to produce magnified visual or photographic images of small objects

A

microscope

51
Q

micrographia is he book that contains all the…

A

principles of the compound microscope

52
Q

who is the first man to discover the cellular structure of cork

A

robert hooke

53
Q

who invented the phase-contrast microscope

A

frits zernike

54
Q

what kind of MC is used to study of colorless and transparent biological materials

A

phase-contrast microscope

55
Q

what MC hat can magnify an image up to 1 million times

A

electron MC

56
Q

what MC uses a single lens for magnification

A

simple MC

57
Q

how many lens does compound MC have to magnify the image

A

objective lens
ocular lens

58
Q

condenser is present in simple or compound MC?

A

compound

59
Q

compound microscope can have up to how many objective lens

A

4

60
Q

dissecting microscope is aka

A

stereoscopic microscope
stereoscope

61
Q

what MC that image appears in 3D

A

dissecting MC

62
Q

dissecting MC is used to study the… of the solid specimens

A

surfaces

63
Q

which MC is used to count platelets

A

phase-contrast MC

64
Q

which MC employs a special condenser

A

dark field MC

65
Q

which MC uses for demonstration of very thin bacteria

A

dark field MC

66
Q

dark field MC is helpful in microbiology in the identification of

A

spirochetes

67
Q

dark field MC has a dark background created by the

A

special condeser

68
Q

what is the source of illumination of electron MC

A

a beam of electrons

69
Q

in electron MC, living specimen is destroyed by

A

the high radiation of electron beam

70
Q

what kind of electron MC produces a 3D image of specimen’s surface feautures

A

scanning electron MC

71
Q

what kind of electron MC produces a 2D image of specimens

A

transmission electron MC

72
Q

ocular is aka

A

eyepiece

73
Q

what are he 2 types of diaphragm

A

disk
iris

74
Q

disk diaphragm is used in

A

old microscope

75
Q

iris diaphragm can regulate

A

the amount of light by adjusting the lever

76
Q

what are the 2 types of brightfield illumination

A

critical illumination
koehler illumination

77
Q

name the type of brightfield illumination
the light source is focused at the specimen

A

critical illumination

78
Q

name the type of brightfield illumination
the light source is focused at the condenser aperture diaphragm

A

koehler illumination

79
Q

magnification color code for scanning

A

red

80
Q

magnification color code for LPO

A

yellow

81
Q

magnification color code for HPO

A

blue

82
Q

magnification color code for OIO

A

white

83
Q

how do we carry the MC

A

one under the base
other at the grasping arm

84
Q

when turning on the MC and opening the diaphragm, make sure that the

A

light intensity dial is on the owest setting

85
Q

use the what to move to center the specimen over the condenser lens

A

stage adjustment

86
Q

use the what to move the objective as close as possible to the slide

A

coarse adjustment knob

87
Q

when images on MC becomes clear, switch the… to make the image even sharper

A

fine adjustment knob

88
Q

when to inspect the component parts of MC

A

before and after use

89
Q

when not use in a long period of time, the MC should be

A

covered or protected from dust

90
Q

do not use what to clean the MC

A

water

2023 SEM 1 ANAPHY (LEC) (15 decks)

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