Microscope Quiz

Question 1

What are cover slips and immersion oil designed with and what are they for?

Answer 1

refractive indices to trap more orders of light i.e. give more light and better image quality

Question 2

Define resolution.

Answer 2

the smallest distance between 2 points on a specimen that can be distinguished as 2 separate entities

Question 3

What part of the microscope is the resolution dependent on?

Answer 3

the numerical aperture and the substage condenser

Question 4

What does a higher numerical aperture allow for?

Answer 4

more orders of light entering objective therefore getting a higher resolution

Question 5

What areas of the microscope are important for proper use?

Answer 5

eyepiece, objective, stage, condenser, aperture diaphragm, and field diaphragm

Question 6

What areas of the microscope are important for cleaning?

Answer 6

eyepiece, objective, stage, and condenser

Question 7

As the lens size gets smaller, what happens to the magnification?

Answer 7

it rises and less light comes through

Question 8

What is the purpose of the condenser diaphragm?

Answer 8

it opens and closes to regulate light coming from the field diaphragm

Question 9

When the aperture diaphragm is closed what happens to the contrast, resolution, light, and focal depth?

Answer 9

contrast is increased, there is a loss of detail in resolution, illumination is decreased, and there is a deeper focal depth

Question 10

What is contrast?

Answer 10

the difference in light intensity between the image and the adjacent background relative to the overall background

Question 11

How can you increase contrast on a microscope?

Answer 11

by closing the aperture diaphragm or lowering the condenser stage

Question 12

What is contrast and glare good for identifying?

Answer 12

foreign bodies, fungal elements, eosinophil granules, crystals, and ectoparasites

Question 13

When initially using the microscope, what should the light be set at?

Answer 13

50%

Question 14

What are the steps of Kohler illumination?

Answer 14

1. focus on slide using 10x objective
2. lower the condenser stage
3. close field diaphragm and aperture diaphragm
4. slowly raise condenser stage until a small circle of light is seen (raise until edges are sharp, center objective view using set screws)
5. open field diaphragm until light fills objective view
6. adjust aperture diaphragm for objective na

Question 15

When examining mites, what is the best thing to do to increase refractivity?

Answer 15

move the condenser stage down

Question 16

When examining stained bacteria, what is the best thing to do to decrease refractivity?

Answer 16

move the condenser stage up

Question 17

What should you do on the microscope to visualize eosinophils, parasites, and other organisms?

Answer 17

close the aperture diaphragm

Question 18

When should you always use a cover slip?

Answer 18

when examining a slide with 40x objective

Question 19

What should you use to clean the lens of a microscope?

Answer 19

Q-tips

Question 20

What should you use to clean the eyepieces?

Answer 20

kim wipes, lens paper, or cotton tipped applicator

Question 21

How should you clean the objectives?

Answer 21

use lens cleaner and lens paper or cotton-tipped applicator

clean surface by rubbing with a circular motion

Question 22

How should you clean the microscope stage?

Answer 22

use general cleanser or lens cleanser and make sure to clean under the slide holder

Question 23

What is the best way to clean the condenser?

Answer 23

remove it from the body of scope and then use a cotton-tipped applicator or kimwipes to clean it

Question 24

How do you clean the field diaphragm?

Answer 24

use a cotton-tipped applicator or kimwipe