Microscopes

Question 1

Simple light microscope

Answer 1

A magnifying lens which has a fixed magnification and uses only one lens

Question 2

Compound light microscope

Answer 2

Uses combination of lenses (objective lens and ocular lens)

Question 3

How much can compound light microscopes be magnified?

Answer 3

1000 - 1500x

Question 4

Can the specimen be alive in light microscopes?

Answer 4

Yes

Question 5

Can the specimen be alive in electron microscopes?

Answer 5

No

Question 6

How much can simple light microscopes be magnified?

Answer 6

10 - 15x

Question 7

Magnification

Answer 7

The increase in an object’s image size compared with the actual size

Question 8

Resolution

Answer 8

Measure of the clarity of an image

Question 9

How can magnification be calculated?

Answer 9

Objective magnification x eyepiece magnification

Question 10

What is a limitation of a light microscope?

Answer 10

Light microscopes cannot provide the details of a small cell’s structure

Question 11

Electron microscope

Answer 11

Microscope that uses beam of electrons instead of light to view cells

Question 12

How much can electron microscopes be magnified?

Answer 12

Up to 100,000x

Question 13

Scanning electron microscopes

Answer 13

Microscopes that study detailed architecture of cell surfaces of which the scattering leads to 3D images

Question 14

Transmission electron microscopes

Answer 14

Microscopes that study the details of internal cell structure

Question 15

How do electron microscopes function?

Answer 15

By super heating a fungsten filament that releases electrons

Question 16

How do scanning electron microscopes function?

Answer 16

They dehydrate specimen and coat them with thin layer of gold that scatters electrons

Question 17

How do transmission electron microscopes function?

Answer 17

Goes through tungsten filament, gets reflected with electromagnetic objective lens and eyepiece lens and hits the phosphor scent screen which we can look at it from

Question 18

Can the specimen be alive in light microscopes?

Answer 18

Yes

Question 19

Parfocal

Answer 19

Lens that stays in focus when magnification is changed

Question 20

Resolution is proportional to…

Answer 20

the wavelength of the illuminating source