Microscopy Flashcards

1
Q

Parameters

A

Magnification, contrast and resolution

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2
Q

Light Microscope

A

Visible light passes through the specimen then through one (simple) or two (compound) glass lenses. Lenses refract light so image of specimen is magnified into eye

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3
Q

Transmission Electron Microscope

A

Beam of electrons is passed THROUGH a thin section and the electrons that pass through fall on photographic film to reveal image of interior of specimen

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4
Q

Scanning Electron Microscope

A

Electrons are reflected off a specimen covering with an electron dense material (gold) to produce 3-D image of SURFACE of a specimen

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5
Q

Visible Light

A

Wavelength, focus with glass lenses, coloured image
Poor resolution=Lower magnification

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6
Q

Electron

A

Particles, focus with electromagnets, B+W image
Good resolution=Higher magnification

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