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Histopathology > Microtomy > Flashcards

Microtomy Flashcards

1
Q

Process of trimming and cutting the tissue block to form uniformly think slices of tissue

A

Microtomy

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2
Q

Most commonly used in microtomy

A

Paraffin embedded tissues

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3
Q

Can cut tissue sections at a pre determined thickness with the use of a knife

A

Microtome

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4
Q

Standard tissue thickness of tissue section

A

4-6 micrometers

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5
Q

It holds the block in place

A

Block holder

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6
Q

Used for cutting serial sections of large blocks of paraffin embedded tissues

A

Rocking microtome

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7
Q

Most used microtome

A

Rotary microtome

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8
Q

Cutting celloidin embedded sections

A

Sliding microtome

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9
Q

Most dangerous microtome

A

Sliding microtome

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10
Q

Used for cutting unembedded frozen sections

A

Freezing microtome

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11
Q

Used for intraoperative procedures

A

Freezing microtome

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12
Q

Used for cutting sections for electron microscopy

A

Ultra thin microtome

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13
Q

Other term for rocking microtome

A

Cambridge microtome

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14
Q

Invented rocking microtome

A

Paldwell Trefall

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15
Q

Known as the simplest microtome

A

Rocking microtome

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16
Q

When using, it will produce a slightly curved plane usually in 10-12 micrometers

A

Rocking microtome

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17
Q

Other name for rotary microtome

A

Minot microtome

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18
Q

Who invented minot microtome

A

Minot

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19
Q

What is the motion in rotary microtome

A

Reciprocal motion

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20
Q

Thickness of the block is automatically regulated by

A

Ratchet feed wheel and the pawl

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21
Q

Who invented the sliding microtome

A

Adams

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22
Q

When to use sliding microtome

A

Celloidein-embedded sections

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23
Q

Used to cut large refractory paraffin blocks

A

Sliding microtome

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24
Q

It is a type of sliding microtome suited for sectioning specimens that are embedded in all forms of media

A

Base-sledge microtome

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25
Q

Who invented the freezing microtome

A

Queckett

26
Q

It is a bench-top type of microtome in which it uses carbon dioxide to freeze the sample

A

Freezing microtome

27
Q

It is a refrigerated apparatus and inside is a microtome

A

Cryostat

28
Q

Temperature maintained in a cryostat

A

-5 to -30 degrees celsius

29
Q

The cryostat freezes the tissues within how many minutes

A

2-3

30
Q

It is utilized for rapid preparations for urgent biopsies

A

Cryostat/freezing microtome

31
Q

How thick is the tissue section in ultrathin microtomy

A

0.5 micrometers

32
Q

Knife used in ultra-thin microscopy

A

Diamond knife

33
Q

The specimen used in ultra-thin microtome is fixed in

A

Osmium tetroxide

34
Q

Recommended side for cutting celloidin-embedded tissue blocks

A

Less concave side

35
Q

Used to cut paraffin embedded section

A

More concave side

36
Q

It is a 25 mm in length ; one side is flat and the other side is concave

A

Plane-concave knife

37
Q

Both sides are concave and is recommended for cutting paraffin embedded sections in rotary microtome

A

Biconcave knife

38
Q

Length of a biconcave knife

A

120 mm

39
Q

Length of a plane wedge knife

A

100 mm

40
Q

A knife that’s both sides are straight and recommended for frozen sections

A

Plane-wedge knife

41
Q

Knife Used for cutting extremely hard and tough specimens

A

Plane-wedge knife

42
Q

The perfect and optimum cutting angle

A

15 degrees

43
Q

To remove the irregularities of the knife

A

Honing

44
Q

Removal of gross nicks on the edge of the knife to remove blemishes

A

Coarse honing

45
Q

Grinding the cutting edge of the knife on a stone to acquire an even edge angle

A

Honing proper

46
Q

It is a natural sharping stone grinding surface

A

Hone

47
Q

Most commonly used hone

A

Belgium yellow

48
Q

Hone that gives more polishing effect

A

Arkansas

49
Q

Hone that is much coarser and is used for badly nicked knives

A

Fine carborundum

50
Q

Makes use of a vibrating frosted glass plate/wheel driven by an electrical motor

A

Mechanical microtome knife sharpener

51
Q

Process whereby the burr formed during the honing is removed

A

Stropping

52
Q

Polish and sharpening the cutting edge of the knife

A

Stropping

53
Q

Best quality horse leather, firmly attached to a solid back to prevent sagging

A

Paddle strop

54
Q

How many stroke are required in stropping

A

40-120 strokes

55
Q

The cutting edge of disposable knives

A

2-4 micrometers thick

56
Q

Knife used for cutting or trimming and/for semi thin sectioning of tissue blocks for electron microscopy

A

Glass knives

57
Q

Mostly and commonly used in ultra thin microtome

A

Diamond knives

58
Q

Cut any type of resin blocks

A

Diamond knives

59
Q

It is brittle and expensive

A

Diamond knife

60
Q

Preferred thickness of clean slides

A

1.0 - 1.2 mm

Histopathology (4 decks)

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