Module 1: Cells (Cell Structure) Flashcards
What is the resolution and magnification of a transmission electron microscope?
Resolution: 0.2nm
Magnification: x500 000
What is the resolution and magnification of a light microscope?
Resolution: 200nm
Magnification: x1500
What is the resolution and magnification of a scanning electron microscope?
Resolution: 0.2nm
Magnification: x100 000
Define Magnification.
The degree to which an image is larger than the object itself.
Define Resolution.
The degree to which it is possible to distinguish between two separate points.
Why is staining required in microscopy?
A lot of biological material is not coloured so details cannot be seen
Some material distorts when they’ve been cut into thin sections
How does staining work in light microscopy?
Stains allow various components of a cell to be distinguished from each other; because different parts will stain different colours; it allows for a greater contrast
Staining in light and electron microscopy.
Light: colours or fluorescent dyes
Electron: metal particles or metal salts
How do you calculate the linear magnification of an image?
Magnification = Image Size/Actual Size
M = I/A
What is the function of the nucleus?
It houses nearly all of the cell’s genetic material which codes for the synthesis of proteins.
It contains chromatin which consists of DNA and protein.
What is the function of the nucleolus?
It makes RNA and ribosomes.
It is the site of the synthesis of ribosomes.
What is the function of the nuclear envelope?
It is the membrane enclosing the nucleus.
It contains nuclear pores which allow content to move in and out of the nucleus. e.g. mRNA leaves the nucleus through nuclear pores to go to ribosomes.
What is the function of the rough endoplasmic reticulum (RER)?
Contains ribosomes on the surface.
Synthesis of proteins by attached ribosomes.
Transport of proteins for modification or secretion.
What is the function of the smooth endoplasmic reticulum (SER)?
Synthesis and transport of lipids.
What is the function of the Golgi apparatus?
Modification and packaging of proteins into vesicles for storage or secretion