Module 10

Question 1

gene expression

Answer 1

the process of turning a gene “on” to produce RNA and ultimately a protein

Question 2

what influences gene expression

Answer 2

epigenome

Question 3

epigentetic tags

Answer 3

turn a gene on and off

Question 4

Reproductive Cloning via Somatic Cell Nuclear Transfer

Answer 4

1. take a somatic cell from the donor
2. de -differentiate the cell
3. another organism donates an egg cell
4. the nucleus is taken from the egg cell
5. the somatic and egg cell are combined
6. the combined cell is placed in a sergout mother to develope

Question 5

Molecular cloning

Answer 5

1. isolate the target gene
2. insert the target gene into a plasmid
3. insert the plasmid into bacteria
4. grow the bacteria

Question 6

Gel electrophoresis

Answer 6

• separates DNA fragments based on size
• DNA has a negative charge
• DNA fragments move through the gel
• The smallest, shortest, lightest fragments travel the furthest

Question 7

CRISPR

Answer 7

a tool used to edit DNA; genetic engineering the utilized a bacterial system
- guide RNA targets DNA of interest
- CAS9 enzyme cuts DNA and edits it