MOUNTING

Question 1

Process whereby a syrupy fluid is applied between the section and the coverslip after staining (handling, storage, and protection of tissue section)

Answer 1

MOUNTING

Question 2

Goal of MOUNTING (3)

Answer 2

1. Protects the stained smear from getting scratched and from bleaching or deterioration due to oxidation
2. Preserves the slides for permanent keeping to facilitate easy handling and storage
3. Promote better refraction for better microscopic visualization

Question 3

COVERSLIP USED:

Answer 3

 Coverslip # 1.5 (180 nm thick): ideal for photomicrography
 Coverslip #1 (150 nm thick) routinely used

Question 4

COVERSLIP USED: ideal for photomicrography

Answer 4

Coverslip # 1.5 (180 nm thick):

Question 5

COVERSLIP USED: routinely used

Answer 5

Coverslip #1 (150 nm thick)

Question 6

Sizes of COVERSLIP USED

Answer 6

18x18 mm, 22x22mm, 20x20mm, 24x24 mm

Question 7

FACTORS TO CONSIDER IN CHOOSING APPROPRIATE MOUNTING MEDIUM: (6)

Answer 7

1. Should have refractive Index close to that of slide= 1.518
2. Should set hard permanent mounting of tissue section
3. Should not dry quickly
4. Should not cost bleaching
5. Should not change the color of the tissues
6. Should not be miscible with xylene

Question 8

-temporary mounting (short term storage)
-usually made up of gelatin, glycerin jelly and glycerol

Answer 8

Aqueous Mounting Media

Question 9

low refractive index, moderately transparent and evaporates easily

Answer 9

Water

Question 10

Standard mounting medium, may be used as a preservative, provides greater
visibility, semi-permanent

Answer 10

Glycerin

Question 11

stains may tend to faide, requires ringing

Answer 11

Glycerin Jelly/Kaisser’s

Question 12

does not require heat before using, but
longer time to harden

Answer 12

Farrant’s medium (Gum Arabic Medium)

Question 13

recommended for Methylene Blue Stained Nerve Preparation

Answer 13

Apathy’s medium

Question 14

for Frozen section, and sections from water

Answer 14

Brun’s Fluid

Question 15

REFRACTIVE INDEX of Apathy’s Medium

Answer 15

1.52

Question 16

REFRACTIVE INDEX of Farrant’s Medium

Answer 16

1.43

Question 17

REFRACTIVE INDEX of Glycerin Jelly/Kaisser’s

Answer 17

1.47

Question 18

REFRACTIVE INDEX of Glycerin

Answer 18

1.46

Question 19

Kinds of mounting medium: (2)

Answer 19

1. Aqueous Mounting Media
2. Resinous Mounting Media

Question 20

-permanent mounting (long term storage)
-recommended for majority of staining method
-used for preparations that have been dehydrated and cleared in xylene and toluene

Answer 20

Resinous Mounting Media

Question 21

natural resin extracted from Canadian tree “Abus Balsamea”
o Usually dissolved in xylene in an incubator @ 37°C or paraffin oven @ 58°C o Recommended for whole mounts and thick sections

Answer 21

Canada Balsam

Question 22

recommended for small tissue sections, not for whole mounts, dries rapidly

Answer 22

DPX

Question 23

synthetic resin mixture dissolved in xylene

Answer 23

XAM

Question 24

synthetic resin soluble in xylene , preferred over DPX

Answer 24

CLARITE

Question 25

REFRACTIVE INDEX of Canada Balsam

Answer 25

1.524

Question 26

REFRACTIVE INDEX of DPX

Answer 26

1.532

Question 27

REFRACTIVE INDEX of XAM

Answer 27

1.52

Question 28

REFRACTIVE INDEX of CLARITE

Answer 28

1.544

Question 29

Other recommended Mountant (3)

Answer 29

 Permount
 Harleco Synthetic Resin (HSR)
 Clearmount

Question 30

Sealing of margins of the coverslip to prevent the escape of fluid or semi-fluid mounts or evaporation

Answer 30

Ringing

Question 31

Kinds of Ringing Media

Answer 31

Kronig Cement
Duroflix/ Du Noyer’s

Question 32

: 2 parts paraffin mixed with 4-9 parts powdered colophonium resin

Answer 32

Kronig Cement:

Question 33

Cellulose adhesives

Answer 33

Duroflix/ Du Noyer’s

Question 34

Process of indicating the year and specimen number on one end of the prepared slide for proper identification

Answer 34

Labelling