Myeloid

Question 1

Flow apml

Answer 1

-ve hla-dr and CD 34
Strong CD 33, heterogenous cd13
CD 117 usually expressed
Aberrant cd2& CD 56 - worse prog

Question 2

Flow acute mast cell leukaemia

Answer 2

CD 13, CD 33 cd 68 cd117

Question 3

M7

Answer 3

Cytoplasmic blebs
Antigène of vwf and CD 42(go 1b) CD 41(go IIb/IIIa) on blasts
Usually dry tap 
common 
Phénotype in Down syndrome

Question 4

What do eukaemic pro myélocytes stain intensely with

Answer 4

Sudan black and mpo

Question 5

Other translocation partners for RAR alpha

Answer 5

5:17- npm-rar fusion
3:17 tblr- rar
Both of these retinoids responsive
11;17 only one that isn’t it- usually lack Ayer rods and has CD 56 exp

Question 6

Monosomal karyotype

Answer 6

two or more autosomal monosomies or a single autosomal monosomy plus a structural abnormality)

Question 7

Which molecular mutations are associated with normal karyotype in aml

Answer 7

NPM1, CEBPA, FLT3-ITD, DNMT3A, IDH1,orIDH2

Question 8

Favourable risks

Answer 8

sFavorablet(8;21)(q22;q22.1); RUNX1-RUNX1T1 
inv(16)(p13.1q22) or t(16;16)(p13.1;q22); CBFB-MYH11
Mutated NPM1 without FLT3-ITD or with FLT3-ITDlow 
Biallelic mutated CEBPA

Question 9

Intermediate risk

Answer 9

Mutated NPM1 and FLT3-ITDhigh allelic ratio 
Wild-type NPM1 without FLT3-ITD or with FLT3-ITDlow allelic ratio (w/o adverse-risk genetic lesions) 
t(9;11)(p21.3;q23.3); MLLT3-KMT2a 
Cytogenetic abnormalities not classified as favorable or adverse

Question 10

Poor risk

Answer 10

t(6;9)(p23;q34.1); DEK-NUP214
t(v;11q23.3); KMT2Arearranged
t(9;22)(q34.1;q11.2); BCR-ABL1
inv(3)(q21.3q26.2) or t(3;3)(q21.3;q26.2); GATA2,MECOM(EVI1)
−5 or del(5q); 
−7; −17/abn(17p)
Complex karyotype
 monosomal karyotype
cWild-type NPM1 and FLT3-ITD high
Mutated RUNX1d
Mutated ASXL1d
Mutated TP53e