PAG 1 Microscopy

Question 1

Function of stage micrometer

Answer 1

To calibrate the eyepiece graticule

Question 2

How is an eyepiece graticule calibrated

Answer 2

Select magnification and objective.
Place stage micrometer on the stage
Line up the scales of the micrometer and the eyepiece graticule
Count the number of Divisions on the eyepiece graticule equivalent to each division on the stage micrometer
Calculate the length of one division of the eyepiece graticule

Question 3

Outline the procedure to preparing a slide

Answer 3

Stain the sample
Mount the sample onto the slide
Place a cover slip carefully on the slide, avoiding air bubles

Question 4

How is magnification calculated

Answer 4

Size of Image / Size of object

Question 5

Differential Staining

Answer 5

Multiple stains are used and each cell binds to a cell structure

Question 6

Stain: Acetic Orcein

Answer 6

Binds to DNA + stains chromosomes red

Question 7

Eosin

Answer 7

Stains cytoplasm dark red

Question 8

Stain: iodine

Answer 8

Stains starch black

Question 9

Wet Mount

Answer 9

Use a pipette to drop water on the slide

Use tweezers to place specimen in water

Stand cover slip upright on the slide and tilt it down

Add a stain