PRE-LAB 1.2 MICROSCOPY

Question 1

Light microscopy

Answer 1

-view entire cell (morphology)
-less magnifying power
- view living cells

Question 2

Simple Light Microscope

Answer 2

• single lens to reflect natural light to the sample slide

Question 3

Compound light microscope

Answer 3

-uses two or more lens to reflect light off the sample slide making the specimen look bigger

Question 4

Dissecting light microscope

Answer 4

• single objective lens, two eyepiece lens
  -used for dissection and 3D specimens

Question 5

Method in light microscopy:
Standard Light Microscopy (brightfield)

Answer 5

Light passes directly through the
specimen. Has a lesser quality of observation
due to unstained samples having lesser
contrast.

Question 6

Method in light microscopy:
Phase Contrast Microscopy

Answer 6

-manipulated by condenser inducing contrast
- better quality but creates halos
-dark parts= denser
-used for observing living unstained cells

Question 7

Method in light microscopy:
Differential Interference Contrast Microscopy (Normanski)

Answer 7

-similar to phase contrast but has plane-polarized light, Normanski prisms
-internal structures

Question 8

Modified prism of made of two merged crystalline quartzes

Answer 8

Wollaston prism

Question 9

Method in light microscopy:
Fluorescence microscopy

Answer 9

-stained samples
-sample appears bioluminescent
-fluorescent signifies cellular protein or organelle