Protein Extraction Of Recombinant Sfgfp From Ecoli Flashcards by hime shiratori | Brainscape

Q

Lysis buffer components

A

Phosphate buffer saline
0.20 mg/ml lysozyme
0.5% (v/v) tween 20
1x sigmafast protease inhibitor

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Q

Sonication prep components

A

2 tubes with 10 ml ddh2O
1 tube 10 ml 70% ethanol

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Q

Supernatant and pellets other term

A

Soluble and insoluble fraction

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