Protein Hydrolysis Flashcards
What are exoenzymes capable of?
Hydrolyzing large proteins.
What metabolic activity is protein hydrolysis?
Polymer hydrolysis
What are large proteins?
Polymers of amino acids.
What are the end products of hydrolyzed large proteins?
Soluble end products…
- Peptones
- Proteoses
- Peptides
- Amino acids
What are the smaller fragments used for after the protein is hydrolyzed?
Used in cell building material or for energy.
What are the two enzymes capable of degrading proteins for this lab?
- Gelatin
2. Casein
Where does gelatin derive from?
A typical animal protein, derived from collagen found in hide and connective tissues of animals.
What type of protein is casein?
An animal milk protein.
At what temperature is gelatin a solid?
20 degrees C (rm temp.)
At what temperature does gelatin melt?
28-30 degrees C
Describe a negative gelatin result (protein is unhydrolyzed).
Gelatin remains, protein precipitates, forming a cloudy white turbidity.
E. Coli was negative.
Describe a positive gelatin test (protein is hydrolyzed)
There will be a well created where the medium was liquified.
Serratia marcescens was positive (turns a bit red).
In reference to casein what is peptonization?
When an organism is capable of digesting casein; causes a clearing of milk…
Describe a positive casein test (protein is hydrolyzed).
There will be a clear zone around the growth.
Bacillius ceresus was a positive test.
Describe a negative casein test (protein is unhydrolyzed).
Area will remain opaque.
E. Colin was negative.
