Proteomics And Mass Spectrometry

Question 1

What is a proteome?

Answer 1

Set of proteins expressed in a cell, tissue, organ or organism at a particular time under specific conditions

Question 2

What is proteomics?

Answer 2

A complete proteome

Question 3

Is the human genome or human proteome bigger?

Answer 3

The human proteome

Question 4

What mechanisms contribute to protein complexity?

Answer 4

Alternative splicing
Post-translational modifications

Question 5

What are the stages of a 2D-DIGE?

Answer 5

1. Stain the two samples with different flurophores
2. Combine the two samples into 1
3. Run IEF followed by SDS-PAGE

Question 6

What are the 2 applications of microarrays?

Answer 6

Analytical protein arrays (mostly antibody arrays)
Functional protein microarray (purified protein)

Question 7

What can functional microarrays be used to identify?

Answer 7

Signalling partners

Question 8

What is mass spectrometry used for?

Answer 8

Identification of proteins

Question 9

What does acceleration depend on for mass spectrometry?

Answer 9

The m/z ratio (mass to charge ratio)

Question 10

What are the steps of mass spectrometry?

Answer 10

1.Sample is introduced
2. Ionisation
3. Mass analyser
4. Detector

Question 11

What parameters can be changed in mass spectrometry to increase the chances of identifying the protein of interest?

Answer 11

Mass accuracy
Number of peptides