quiz 1

Question 1

magnification

Answer 1

factor by which an image is magnified. on compound microscopes, this is calculated by multiplying the power of the ocular (eyepiece) times the objective (nosepiece)

Question 2

compound scope max magnification power

Answer 2

1000 X bc of limitations of resolving power

Question 3

resolving power

Answer 3

the ability to distinguish two objects as separate, calculated as the distance between the two objects.

Question 4

max resolution on compound scopes

Answer 4

0.2 picometers

Question 5

contrast

Answer 5

the apparent difference of images against a background

Question 6

how do you increase the contrast of an image

Answer 6

add dyes to the observation

Question 7

what helps with the light diffraction

Answer 7

closing the iris

Question 8

phase contrast scopes

Answer 8

transform phase shifts caused by light passing through different objects into differences in light intensity, produces high contrast in image and improves resolution, includes Nomarski Process

Question 9

transmission electron scopes

Answer 9

transforms differences in the stream of electrons passing through different objects into differences in light intensity, requires staining of the specimen with heavy metal ions, vastly improves resolution power, magnification

Question 10

scanning electron microscopes

Answer 10

electron beams directed upon gold or platinum plated surfaces cause scattering of additional electrons, the more direct the scattering, the higher the intensity, giving surfaces a shiny metallic, 3-d appearance.