Quiz 5 Flashcards
What is the purpose of southern blot experiment?
To study the presence of one gene in multiple species
Steps of southern blot
- collect in low DNA in different species in different wells of agarose gel
-Electrophoresis
-Transfer DNA to nitro cellulose paper
-Add nuclei acid probe - Label fluorescent radioactive
- chemiluminescent
- look at the bands
What is the point of northern blot?
For the expression of one gene for different cells of the same species or different species
Steps for northern Blot
- collect and load RNA in different species and different wells of agarose gel
- run electrophoresis
- Blood transfer DNA to nitro cellulose paper
- add nuclei acid probe
- if you see bands on the block, gene expression is expressed
What is rRNA?
Ribosomal RNA combined with ribosomal proteins will give you ribosomes
How many genes are in rRNA processing
Two genes: 45s pre rRNA & 5s pre rRNA
Where does DNA to rRNA occur?
Nucleolous
Steps to rRNA processing
-transcription will occur first
-Then ribosomal proteins bind 45 pre-rRNA
-Chemical modifications to pre-RRNA
-Add methyl to basis and sugars to nucleotides and change urine
-Cleve Pre rRNA into 18s, 5.8s, 28s
-generate pre-ribosomal particles (small subunit &large subunit)
- pre-ribosomal particles, exit nucleus, and mature in the cytoplasm
What is the smallest sub unit made of
18 S and proteins
What is the large Saub unit unit made of
5.8 S, 28S, 5S, and proteins
The large subunits has how many proteins
49
The smallest unit has how many proteins
33
What are the names of the sites in the large Subunit
A site P site E site
What is the A site stand for and what happens there?
Aminoacyl site: second tRNA with amino acids and all subsequent tRNA with amino acids will enter
What does the p site stand for and what happens there?
Peptidyl site: site for peptide bond formation
