quiz2

Question 1

What types of culture media are there?

Answer 1

Broth and solid

Question 2

What is broth media?

Answer 2

liquid

Question 3

What is solid media?

Answer 3

Broth + agar

Question 4

What are 2 positive characters of agar?

Answer 4

1. Solid up to 65 degrees del

2. bacteria cannot break down agar

Question 5

If a media is complex, it has:

Answer 5

Crude ingredients; exact amount of each element. Ex: beef extract

Question 6

If a media is defined; it has

Answer 6

Purified chemicals; exact amount of each element is known.

Question 7

define culture media

Answer 7

Mix of materials to grow microbes in vitro (in glass/plastic)

Question 8

What are the 4 basic components of culture/media

Answer 8

wcnm: Water, carbon, nitrogen, minerals

Question 9

What are 2 reasons why you would streak a plate of bacteria?

Answer 9

1. Determine if you have a pure culture

2. Obtain pure culture or maintain pure culture

Question 10

What is the field of view on the microscope

Answer 10

Lit area around specimen

Question 11

What happens to the field of view as the magnification increases with the microscope

Answer 11

The field of view decreases

Question 12

Define what it means for microscopes to be parfocal

Answer 12

When switching objectives, only minimal focusing is required

Question 13

What two things is the resolution affected by

Answer 13

1. Len quality.

2. Amount of light

Question 14

What is the working distance of a microscope

Answer 14

The space between the objective, as magnification increases our working distance goes down

Question 15

What is the stage of micrometer of the microscope

Answer 15

A millimeter ruler etched into a glass side

Question 16

What two ways can you prepare illustrations of what you saw through the microscope

Answer 16

1. illustrations of two different samples

2. The same sample at two different magnifications

Question 17

What are the units of the ocular micrometer

Answer 17

Lines. It’s arbitrary

Question 18

Define two examples of aseptic technique

Answer 18

1. preventing infection of people

2. Preventing contamination of culture

Question 19

What are buffers and what do they do

Answer 19

Stabilizes pH to prevent pH change

Question 20

Define direct stain

Answer 20

Stains the cells

Question 21

Define differential stains

Answer 21

Different cell types will appear differently, even though treated exactly the same

Question 22

Define Gram stain

Answer 22

Two colors, pink or purple, it depends on cell wall thickness

Question 23

If the cell is Graham positive it is

Answer 23

Purple and has a thick cell wall

Question 24

If the cell is gram-negative it is

Answer 24

Pink, thin cell wall

Question 25

If the cell has no velocity from the KOH test it is

Answer 25

Gram-positive, has a thick walls

Question 26

If I cell as velocity from the KOH test it is

Answer 26

Gram-negative, the cell has thin walls

Question 27

What are the 3 forms of a culture to grow a bacteria on?

Answer 27

Petri plate. Deep tube. Slant tube

Question 28

What are the units of the stage micrometer?

Answer 28

Millimeter and micrometer

1mm= 1000 microns

Question 29

What do you direct stains do

Answer 29

Dye attaches to cells

Question 30

What do you indirect stains do

Answer 30

Dye does not attached to cell, it stains the background