Storage and Retrieval of Genetic Information Flashcards Preview

Molecules to Man > Storage and Retrieval of Genetic Information > Flashcards

Flashcards in Storage and Retrieval of Genetic Information Deck (14):
1

What are the differences between RNA and DNA

DNA = H on C2 = stable
RNA = OH on C2 = Can attach to phosphate causing denaturation of 2 RNA molecules. Not good long term store of information

2

What is upstream and downstream

3 prime = downstream
5 prime = upstream

3

What are genes

Structural information coding for a protein

4

What are Gene regulatory elements

Promotor = close to gene
Enhancer = further way from gene
Bind to specific transcriptional activators

5

What are non-coding DNA

Exon = coding
Intron = non-coding
Introns are removed from primary DNA in RNA

6

What are the building blocks for DNA synthesis

Deoxynucleoside trisphosphate

7

What direction does DNA replication occur in

5 prime to 3 prime

8

What do primers have that allow DNA replication

Free 3 primes to receive dNTPs

9

Where does the energy come from

Hydrolysis of trisphosphate to 1 when dNTP 5 prime added to 3 prime

10

What is the difference between leading and lagging strand

Leading strand = continuous synthesis
Lagging strand = discontinuous synthesis. Okazaki fragments made then back-stitched together

11

What does DNA polymerase do

Proof reads code

12

What is the building block for transcription

Nucleoside trisphosphate

13

What is the splicesome

Enzymes responsible for splicing on RNA polymerase then jump on RNA transcript to remove introns

14

How many reading frames are there for the genetic code

3