Subcellular Fractionation

Question 1

Avoids extrem pH and osmotic pressure and high temperature; employs aqueous solution; Temperature= O-4degrees Celsius; to avoid loss of biologic activity

Answer 1

(1) Extraction

Question 2

Distrups the cell to liberate its contituents; resulting suspension contains intact organelles known as homogenate

Answer 2

(2) Homogenization

Question 3

Manually operated or motor driven pestle is rotated within a glass tube containing the minced fragments of the organ under and a suitable medium

Answer 3

(2) Homogenization

Question 4

Done at successively greater speed, each yield a pellet and supernatant

Answer 4

(3) Centrifugation

Question 5

3 Pellets of Centrifugation

Answer 5

Nuclear fraction, mitochondrial fraction, microsomal fraction

Question 6

Process of isolating specific organelles in relatively pure form, free of contamination by other organelles

Answer 6

Subcellular Fractionation