Terms Nucleic Acids

Question 1

Nucleosides

Answer 1

are bases with sugars

Question 2

Nucleotides

Answer 2

bases sugars and phosphates

Question 3

where are phosphates attached

Answer 3

2,3,5 ‘ OH’s

Question 4

where is the leucine zipper bound

Answer 4

the major groove

Question 5

Where purified RNA stored

Answer 5

in buffer soln like EDTA that have a chealting agent

Question 6

DNA sequencing

Answer 6

can have small amts and use PCR to amplify
heat to melt then cool to anneal

need DNA polymerase

5’ to 3’ synthesis by linking the 5’ to the 3’ OH on the growing chain

Question 7

capillary gel eletrophoresis

Answer 7

the automated seperation where you load it into a gel filled glass capilary

the shortest chain arrives at the detector first and the wave length determines the seq

orginal seq is complemtary tp te graph

Question 8

A dye

Answer 8

560

Question 9

C dye

Answer 9

595

Question 10

G dye

Answer 10

535

Question 11

T dye

Answer 11

620

Question 12

what is the 5’ OH protected as and how is it removed

Answer 12

DMT (more acid-laibe than the polymer/ester so it wont break that)

removed with TCA

Question 13

3’ end protection

Answer 13

using a polymer using a COOH group using DCC forming an ester