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Micro Lab > Tests > Flashcards

Tests Flashcards

0
Q

Bile Esculin test

A
  • tests for breakdown of Esculin
  • slant /stab a streak
  • positive: dark brown or black
  • negative: no color change
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1
Q

B-galactosidase

A
  • presence of b-gal
  • b-gal breaks down ONPG
  • positive yello
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2
Q

CAMP Test

A
  • tests for CAMP factor group B strep.
  • straight line down the middle of plate is stap aureus. Perpendicular line of the organism testing. Do not touch!
  • positive: arrow head ( hemolysis)
  • negative: no increased hemolysis
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3
Q

Catalase test

A
  • tests for the presence of catalase
  • h2o2 -> h2o and o2
  • positive: bubbles
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4
Q

Citrate test

A
  • tests for citrate utilization as only carbon source
  • use Simmons-citrate tubes. Streaks lank. Incubate with loose cap
  • positive: blue
  • negative: still green
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5
Q

Gelatin hydrolysis

A
  • tests for the presence of gelatinase
  • stab tube of gelatin
  • positive: liquid
  • negative: solid
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6
Q

Gram stain

A
  • test for gram+ or gram- cells
  • crystal violet stains
  • positive: purple
  • negative: pink
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7
Q

Hemolysis

A

Gamma- no hemolysis, no clearing
Alpha- partial hemolysis ( green or yellow)
Beta- complete hemolysis ( transparent)

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8
Q

Kirby- Bauer test

A
  • test for antibiotic efficacy
  • use mueller-Hinton plate
  • zones of inhibition
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9
Q

Lipase test

A
  • tests for lipase/ breakdown lipids
  • spirit blue plate. Multi stab
  • positive: white clearing
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10
Q

MacConkey agar

A
  • tests for inhibition growth of gram+, differentiate enterococcus
  • lactose fermentation
  • positive: pink ( lactose fermentor)
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11
Q

Mannitol Salt agar

A
  • test for differential for gram+ organisms
  • phenol Red indicator and mannitol
  • yellow= indicates organism ferments
  • mannitol= only gram+ species grow
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12
Q

Methyl Red VP

A
  • tests fir mixed acid ferment of glucose
  • positive: red
  • negative: yellow
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13
Q

Nitrate reduction

A
  • tests for reduction of nitrogen and gas production
  • add 5 drops of A &B reagent. If no red after 5 min add reagent C
  • positive: red for nitrate -> nitrite
  • negative: no change
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14
Q

Oxidase test

A
  • test for presence of cytochrome oxidase
  • pos. organism use oxidative phosphorylation
  • positive: purple/ blue
  • negative: no color change
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15
Q

Phenylalanine deaminase test

A
  • tests for presence of phenylalanine deaminase enzyme
  • slanted tube- streak slant, add ferric chloride after incubation
  • positive: dark green
  • negative: no change
16
Q

SIM agar/ indole

A
  • test for motility, sulfur reduction, and indole production using tryptophanase
  • SIM agar, straight stab
  • indole produced if red after rgt added
  • motility = bacteria diffuse from stab
  • black = reduction of sulfur
17
Q

Starch hydrolysis

A
  • Tests for presence of hydrolase
  • alpha-amylase cuts starch into amylose & amylopectin
  • positive: clearing of iodine
  • negative: no clearing
18
Q

Sugar fermentation

A
  • test for utilization of carb
  • phenol red ( ph indicator)
  • positive: yellow
  • negative: red
19
Q

Triple sugar iron (TSI)

A
  • test for carb utilization and production of h2s
  • phenol red, lactose, glucose, sucrose,mans ferrous sulfide
  • yellow butt = only glucose
20
Q

Urease test

A
  • test for presence of urease enzyme
  • slanted tube- stab and streak
  • positive: pink
  • negative: yellow
21
Q

Vogel- Johnson agar

A
  • test for selective and differential for staphylococcus species
  • mannitol carbon source and potassium tellurite
  • yellow ferments mannitol
  • dark grey = reducing potassium tellurite
22
Q

Voges-Proskaure test

A
  • test for 2,3-butanediol fermentation -> presence of acetoin
  • 15 drops VPA, 5 drops VPB, Shake, results in 10-15 minutes
  • positive: red ( acetoin present)
  • negative: no color change
23
Q

Acid-fast stain

A
  • differential stain which distinguishes organisms with waxy cell walls that can resist decolonization with acid alcohol
  • use heat to drive in stain
  • acid-fast cells will be red/pink
  • non acid-fast cells are blue
24
Q

Spore stain

A

Spore= very tough coat ( only one per bacterium)

  • environmental stress triggers production
  • spore is green
  • bacteria red
25
Q

Capsule stain

A
  • have mucodial, shiny colonies
  • firm gelatinous layer
  • capsule the more virulent
    Capsule is white
    Bacteria is purple
    Background pink
26
Q

Flagella stain

A
  • visualization of flagella
  • mordant helps build stain on flagella
  • see as purple
27
Q

Fluid thioglycolate test

A
  • tests the oxygen requirements
  • contains sodium thioglycolate
    Obligate anaerobes- growth on bottom of tube only
    Obligate aerobe- growth only on top of tube
    Aerotolerant- uniform growth top to bottom
    Facultative- uneven distribution (more at top)
28
Q

IMViC test includes

A

Indole, methyl red, voges-Proskaure, and citrate

Micro Lab (1 decks)

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