Topic 4 - Genetic info, variation and relationship between organisms Flashcards
Give 2 structural differences between tRNA and mRNA
- tRNA is cloverleaf, mRNA is linear
- tRNA has hydrogen bonds, mRNA doesn’t
- tRNA has anticodon, mRNA has codons
- tRNA has amino acid binding sites, mRNA doesn’t
Describe and explain how pre-mRNA and mRNA are different
pre-mRNA has introns
due to splicing
Describe how one amino acid is added to a polypeptide that is being formed at a ribosome during translation
- tRNA brings specific amino acid (to ribosome)
- Anitcodon binds to codon
- Amino acid join by condensation reaction, using ATP
Describe how mRNA is produced from an exposed template strand of DNA
- nucleotides (free RNA) form complementary base pairs
- phosphodiester bonds form
- by DNA polymerase
Describe how mRNA is formed by transcription in eukaryotes
- Hydrogen bonds break
- Only one DNA strand acts as a template
- Free RNA nucleotides align by complementary base pairing
- Uracil pairs with adenine
- RNA polymerase joins adjacent RNA nucleotides together
- Phosphodiester bonds
- pre-mRNA is spliced to remove introns
Describe how a polypeptide is formed by translation of mRNA
- mRNA attaches to ribosome
- tRNA anticodons bind to complementary mRNA codons
- tRNA brings specific amino acid
- Amino acids joins by peptide bonds
- with use of ATP
- tRNA released
- ribosome moves along the mRNA to form a polypeptide
Describe the role of a ribosome in the production of a polypeptide
- mRNA binds to ribosome
- Idea of two codons
- tRNA with anticodons bind
- catalyses the formation of peptide bonds between AMINO ACIDS
- moves along
Describe how mRNA is produced in a plant cell
- DNA strands separate by breaking the hydrogen bonds
- only one strand acts as a template
- complementary base pairing
- (RNA) nucleotides joined by RNA polymerase
- pre-mRNA formed
- splicing removes introns
What is meant by degenerate?
More than 1 codon for each amino acid
What is meant by proteome?
Full range of proteins that a cell can produce
Describe how a gene is a code for the production of a polypeptide
- Base sequence
- In triplets
- Determine the sequence of amino acids
Define exon
Base sequence coding for a polypeptide
Describe how a phosphodiester bod is formed between 2 nucleotides within a DNA molecule
- condensation reaction
- between the deoxyribose and phosphate
- catalysed by DNA polymerase
Define non-coding and where the non-coding multiple repeats are positioned in genes
- DNA sequence that doesn’t code for a protein
- positioned between genes
Give 3 ways in which DNA in chloroplasts is different from DNA in the nucleus
- shorter
- not associated with histones
- no introns
Give a difference between RNA and DNA
- RNA- ribose whereas DNA- deoxyribose
- RNA- uracil whereas DNA- thymine
Compare and contrast the DNA in eukaryotic cells and prokaryotic cells
BOTH- phosphodiester bonds, same nucleotide structure
CONTRAST- E- longer P- shorter
E- introns P- no introns
E- DNA is associated with histones P- DNA is not associated with histones
E-linear P-circular
What is a homologous chromosome?
Chromosomes that carry the same genes in the same loci