Translation

Question 1

What are the 2 important eIFs in eukaryotic translation initiation?

Answer 1

eIF4E- Cap binding protein

eIF2-alpha: binding initiator tRNA to ribosome

Question 2

What is unique about the process by which eukaryotes initiate translation?

Answer 2

Scanning by the small subunit of the ribosome on the mRNA

Question 3

Which elongation factor is responsible for translocating the mRNA and tRNA?

Answer 3

EF2

Question 4

Which elongation factor is responsible for bringing a tRNA into the A-site of a ribosome (pro and eukaryotes)?

Answer 4

EF1A in eukaryotes

EF-Tu in prokaryotes

Question 5

How many high energy molecules are required to complete a cycle in a ribosome?

Answer 5

4
2-Charge AA (ATP)
1- Deliver to A-site (GTP)
1- Translocate (GTP)

Question 6

What causes termination and ribosome dissociation?

Answer 6

Release factors binding to stop codon.

Question 7

What is IRES?

Answer 7

Cap independent initiation or (Internal Ribosomal Entry Site)

Question 8

What causes Hemoglobin Wayne?

Answer 8

Frame shift mutation

You get punched by Bruce Wayne and your frame shifts

Question 9

What causes Hemoglobin Constant Spring?

Answer 9

Sense mutation (loss of stop codon)

Question 10

Under high levels of Fe in the cell, how are the proteins Ferritin and Transferrin Receptor affected?

Answer 10

Ferritin stores Fe so it is unregulated.

Transferrin Receptor brings Fe into the cell so it is downregulated.