Urine Microscopic Examination

Question 1

Specimen should be examined while ___ or ______

Answer 1

fresh or adequately preserved

Question 2

Required Refrigeration for Specimen Preparation are:

Answer 2

Precipitation of amorphous urates/phosphates, other non-pathologic crystals

Question 3

may dissolved some crystals

Answer 3

Warming @37degC

Question 4

Standard amount of urine

Answer 4

Usually 10 and 15mL
12mL is frequently used

Question 5

Note to the report for specimen volume

Answer 5

If the urine volume is not adequate,
especially in pediatric patients

Question 6

Centrifugation time for urine

Answer 6

5 minutes at 400 RCF *may vary at other hospitals or lab

Question 7

Volume for Sediment preparation

Answer 7

0.5 mL and 1mL are frequently used
(uniform amount of urine sediment)

Question 8

Volume of sediment examined

Answer 8

Conventional Glass Slide Method
* Recommended volume is 20uL (0.02 mL)
covered by a 22 x 22 mm glass cover slip

Question 9

detects casts, ascertain the general composition of sediment

Answer 9

LPO

Question 10

identification of urinary sediments

Answer 10

HPO

Question 11

Casts have a tendency to locate near the edges of the coverslip (Low power scanning of the coverslip perimeter is recommended. Start with low brightness)

Answer 11

Conventional Glass Slide Method

Question 12

Point of reference

Answer 12

Epithelial cells (big in size)

Question 13

Average number per LPF

Answer 13

Casts

Question 14

Average number per 10 HPF

Answer 14

RBCs and WBCs

Question 15

Semi-quantitative (Rare, Few, Moderate, Many or 1+, 2+, 3+. 4+)

Answer 15

Epithelial cells, Crystals and other
sediments