UV-Vis Flashcards

1
Q

What is Beer-Lambert Law

A

Amount of energy absorbed by a solution is proportional to the path length and concentration

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2
Q

How to get a reliability and best practice

A

UV-Vis spectroscopy experiments and reading will be repeated

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3
Q

How to measure the transmittance

A

Divide the intensity spectrum of light transmitted through through a sample by intensity spectrum of light through the blank

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4
Q

What is absorbance

A

optical density, amount of light absorbed by the object and can be expressed as follows
A=-log (T)

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5
Q

ULight scattering

A

Light scattering often caused by the suspended solid in liquid samples, which may cause serious sample will scatter light, resulting in irreproducible results

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