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Micro Lab > week 2 > Flashcards

week 2 Flashcards

1
Q

complex rich medium

A

variety and surplus of nnutrients that will allow many different bacteria to grow. all media selective to a certain extent.

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2
Q

selective media

A

designed to promote growth of desired bacteria while inhibiting the growth of undesired bacteria.

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3
Q

selective medium isolate desired organism =?

A

enrichment culture

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4
Q

differential media

A

not selective. metabolic activity indicated by coloured reaction.

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5
Q

lactose broth?

A

liquid medium used to show organism can ferment. acid turns phenol red yellow

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6
Q

EMB

A

eosin methylene blue agar - contain lactose + differentiated between lactose fermenters based on their acid production. differential for type of g- but selective for gram negative too becasue dye will kill g+ bacteria

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7
Q

how to label plate

A

what was inoculated with. name initial. TA name, date of inoculation.

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8
Q

what is catalase test?

A

test for presence of catalase. catlse protects cell from H2O2. .

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9
Q

bile-esculin test

A

capable of growth in in presence of bile _ esculin. glycoside found in several plants.
esculin hydrolysis = glucose. esculetin + ferric salt = dark brown-black colour. colour change =+.

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10
Q

BAP test

A

exoenyme = hemolysins rupture rbc membrane. ability to perform hemolysis = key test.
a-hemolysis: formation of greenish zones - oxidation of iron atom due to H2O2. not true.
b-hemolysis: clearing around colonies due to lysis of rbc = true hemolysis
y-hemolysis: no clearing, dicoloration.

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11
Q

MSA

A

mannitol salt agar. .5% NaCl, mannitol + phenol red. selective for staphylococci, pathogenic ferment mannitol. turn medium yellow when pH lower than 6.8.

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12
Q

oxidative test

A

differentiate enteric g- acteria. test for presence of cyt c oxidase. = essential to obligate aerobes. reduced TMPD (-) for cyt c = purple. TMPD oxidied = cyt c + = blue rapidly.

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13
Q

ONPG test

A

hydrolyze lactose. use analog of lactose as substrate for b-galactosidase. ONPG hydrolysis = yellow.

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14
Q

urease test

A

hydrolyze urea to provide nitrogen source to cell.more ammonia = dissolves to base = pH indicator turns reddish/purple.

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15
Q

DMCA test

A

enzyme tryptophanase. convert it to insolde + serine. lyse cells - indole react with DMCA to be blue-green

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16
Q

EMB agar

A

selective and differential medium contains dye to inhibit g+. black metallic = e coli. mixed acid fermentation. butanedial fermentation = e. aerogenes. fish-eye.
lactose non-frmenter: pale/colourless proteus vulgaris.

17
Q

Macconkeys

A

selective contains crystal violet to inhibit g+. contains NaCl and bile to inhibit non-enteric bacteria.
lactose fermenter => acid + neutral pH = red/pink color to colony. e.coli crisp red. e aerogenes pink, gelatinous

18
Q

E. faecalis - plates and results?

A

BAP : a(green) or y (no) hemolysis

MSA : yellow

19
Q

S. aureus - plates + results?

A

BAP: b-hemolysis

MSA: yellow

20
Q

S. epidermidis - plates + results?

A

BAP: y (no) hemolysis

MSA: red medium

21
Q

E. coli - plates + results?

A

EMB: green metallic

MAC: red colonies

22
Q

E. aerogenes - plates and results?

A

EMB: fish-eye

MAC: red gelatinous colonies

23
Q

P. vulgaris - plates and results?

A

EMB: small, pale, colourless.

MAC: colourless

24
Q

tests for g+

A

catalase
bile-esculin
BAP
MSA

25
Q

g- tests

A 
oxidase
catalase
ONPG, Urease, DMCA
EMB
MAC
26
Q

scheme for S. aureus ID

A

catalase (+)
BAP - b-hemolysis
MSA: yellow

27
Q

scheme for S. epidrmidis

A

catalase (-)
BAP: y-hemolysis
MSA: red

28
Q

scheme for E. faecalis

A

catalase (-)
Bile esculin (+)
BAP: a/y = not true.
MSA: red

29
Q

scheme for P. vulgaris

A 
Oxidase (-)
ONPG (-)
Urease (+)
DMCA (+)
EMB = colourless
MAC = colourless
30
Q

scheme for E. coli

A 
oxidase (-)
ONPG (+)
Urease (-)
DMCA (+)
EMB: green, metallic
MAC: red
31
Q

scheme for E. aerogenes

A 
Oxidase (-)
ONPG (+)
Urease (-)
DMCA (-)
EMB: fish eye
MAC: pink slimey

Micro Lab (4 decks)

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