(09) Arteriviridae

Question 1

The four kinds of arteriviridae (which are of the order nidovirales)

1. Porcine Reproductive and Respiratory Syndrome Virus
2. Equine Arteritis Virus
3. Simian Hemorrhagic Fever Virus
4. Lactate Dehyrdogenase-elevating Virus

*SHE IS GIVING US SLIDES ON LAST TWO BUT DOESN:T EXPECT US TO KNOW ABOUT THEM

Question 2

(Characteristics of Arterivirus)

1. Enveloped or no?
2. smooth or rough?
3. shape?
4. Has a tropism for what?

Answer 2

1. enveloped
2. smooth
3. spherical
4. macrophage

Question 3

(Genome)

1. genetic substance?

Answer 3

1. linear, single stranded + sense RNA (has 5’cap and Poly A tail)

(it immediately starts replicating non-structural proteins then moves on to genome)

Question 4

(Genome organization)

1. nonstructural part - has own what to provide proteins for replication?
2. Structural - has overlapping what?

Answer 4

1. proteases
2. reading frames - allows for more proteins with less info

Question 5

(Characteristic of Arterivirus)

1. HAve restricted host range - are they zoonotic?

Answer 5

1. no

Question 6

(Characteristics of Arteriviruses)

1. How transmitted?
2. do they mutate alot?
3. Why is it important that they infect macrophages?
4. Do they causes persistent/asympotmatic diseases or severe/fatol ones?

Answer 6

1. contact/aerosol
2. yep (infidelity of polymerase/ recombination)
3. get past immune responses
4. can be either

Question 7

Answer 7

D

Question 8

(PRRSV)

(Porcine Reproductive and Respiratory Syndrome Virus)

1. How many genotypes?
2. endemic in most swine producing regions worldwide

Answer 8

1. 2

Question 9

(PRRSV)

just read through these

Question 10

PRRSV disease

1. Respiratory Disease in pigs of all ages - most severe in what though?
2. Reproductive disease in pregnant sows - worst when?
3. weaken defenses against secondary infections?

Answer 10

1. growing pigs
2. third trimester (abortions, stillbirths, mummified piglets, weak piglets)
3. yes

Question 11

give this a look

Answer 11

and this

but don’t remember any of these

she just wants us to know that there can be more than one pathogen at once

Question 12

(Route of PRRS)

basically remember that it goes where pigs go - established by contact

Question 13

(PRRSV transmission)

1. stability in environment?
2. Aerosol transmission documented over what distance?

Answer 13

1. relatively unstable (enveloped), can survive in cold
2. 9 km

Question 14

(PRRSV diagnosis)

just read this - sorry seems like a waste of time to ask a bunch of questions about these when

Question 15

(Tools for control and elimianation of PRRS)

1. What are the two ways to combat PRRS?

Answer 15

1. management and immunity

Question 16

1. What are the best condidtions for PRRSV virus to spread?
2. What are put up to reduce spread?

Answer 16

1. cold moist air
2. putting up big filters at points of entrance

Question 17

(PRRSV vaccines)

1. use killed or modified?
2. Which induces better protection
3. Can MLV persist in pigs and be transmistted?
4. Can it revert to virulence?

Answer 17

1. both are used
2. MLV
3. yes
4. maybe

Question 18

Question 19

1. Is PRRSV amenable to eradication?

Answer 19

1. not really - it takes a lot money to do this and a lot of compliance
2. Pigs immune system can eliminate PRRSV - but it takes a long time

Question 20

(Equine Arteritis Virus)

(EAV)

1. What is te tropism for?
2. then move where?
3. syptmoms>
4. worldwide?

Answer 20

1. alveolar macrophages and endothelial cells
2. draining lymph nodes and systemic
3. usually asymptomatic
4. yep

Question 21

(EAV)

1. Mostly subclinical?
2. When you do get signs in adult horses what do you get?
3. what in young foalds?
4. pregnant mares?
5. a vascular injuty, increase permeabtiliy, proinflammatory cytokines

Answer 21

1. yes
2. respiratroy illness
3. pneumonia
4. abortion

Question 22

take a look

Question 23

(Transmission of EAV)

1-2. What are the two routes of transmission?

3. What one is the biggest problem?

Answer 23

1. respiratory/aerosol from acutely affected horses
2. veneral from acutely or chronically infected stallions
3. the carrier stallions

Question 24

(EAV)

1. Does infection induce long lasting immunity?
2. Neutralizing antibodies following infection last how longs?
3. But - virus still persists where?

Answer 24

1. yes
2. years
3. repro tract of carrier stallion

Question 25

(EAV diagnosis) just read this lots of economic loss due to EAV!!!

Question 26

(EAV control) 1. important to identify what? 2. can only breed to what?

Answer 26

1. carrier stallions (test the semen) 2. immune mares separated from suscpetible

Question 27

(EAV vaccines) just read this 1. are there marker vaccines for this?

Answer 27

1. no

Question 28

Answer 28

E

Question 29

just read the remaining stuff (didn't sound like there would be any questions on it)

Answer 29

1. mice

Question 30

know this shit!!!

Answer 30