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Question 1

how many percent of red cell suspension are universally employed indicator to display such reactions?

Answer 1

2%-5%

Question 2

how many rpm when centrifuging in packing or red cells

Answer 2

3,400 rpm

Question 3

what solution is used in removing the supernatant?

Answer 3

10% hypochlorite solution

Question 4

what do you call the process of packing RBCs

Answer 4

washing

Question 5

what does yellowish appearance indicates?

Answer 5

incomplete washing

Question 6

washing removes all traces of what substance?

Answer 6

plasma and other non-red cell entities

Question 7

what is the chemical name of NSS?

Answer 7

sodium chloride

Question 8

why should you use NSS in washing red cells?

Answer 8

to remove serum/plasma which may contain proteins that may affect results, and it removes fibrinogen to avoid clots

Question 9

Why is a Pasteur pipette used in delivering 0.1 ml of washed packed red blood cells instead of a serological pipette?

Answer 9

Pasteur pipette was used to avoid clogging if pipette used has a small bore and will affect the fixed volume.

Question 10

Why is it necessary to wash red cell with NSS for three times?

Answer 10

to reduce allergic reactions due to contaminating plasma proteins

Question 11

What is the important of preparing a red cell suspension in the laboratory?

Answer 11

Red cell suspensions provide the appropriate serum to cell ratio to allow for grading and interpretation of tests results.