1 | Introduction Flashcards

(51 cards)

1
Q

What is the sample to result pathway in clinical biochemistry?

A

Patient –> Sample (Specimen) –> Biochemical Test (Reaction) –> Result

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2
Q

In the sample to result pathway in clinical biochemistry, the role of the pharmacist is…

A

to interpret the result.

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3
Q

Clinical biochemistry is best defined as…

A

the branch of laboratory medicine in which chemical and biochemical methods are applied to the study of disease.

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4
Q

Clinical pharmacy differs from clinical biochemistry in that it…

A

provides drugs for disease treatment.

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5
Q

The adjective “clinical” describes (…) and (…).

A

the practical observations and treatment of patients.

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6
Q

Biochemistry is the science that studies…

A

the various molecules that occur in living cells and organisms with their chemical reactions.

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7
Q

Clinical biochemistry is important for pharmacists to learn because…

A

1) Essential for effective communication and interaction with other healthcare professionals.
2) Pharmacists must have a comprehensive background of the terminology and abbreviations used in the clinical lab context.
3) Pharmacists must understand the basis of lab tests for the monitoring of drug therapy.
4) Pharmacists must understand the influence of disease states and drug therapy on diagnostic lab test results.

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8
Q

The sequence and place of clinical biochemistry in medicine is…

A

History –> Clinical Examination –> Diagnostic Services –> Laboratory Services –> Clinical Biochemistry.

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9
Q

Core biochemical tests are…

A

routinely done and commonly requested.

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10
Q

Specialized biochemical tests are…

A

specific and require special laboratories.

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11
Q

A once-specialized biochemical test that became a core biochemical test is….

A

Lipids and protein profile.

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12
Q

Enumerate the five uses of biochemical tests.

A

1) Disease diagnosis.
2) Treatment monitoring.
3) Disease screening.
4) Assessing the prognosis.
5) Research purposes.

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13
Q

Urgent tests are…

A

analyzed more quickly than others.

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14
Q

The easiest and most non-invasive specimens used for biochemical analysis are…

A

Urine and feces.

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15
Q

What are the three main types of blood specimens?

A

1) Capillary blood.
2) Arterial blood.
3) Venous blood.

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16
Q

Capillary blood must be…

A

used immediately.
(whole blood is obtained by finger puncture)

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17
Q

The blood specimen that is specific for blood gas measurements is…

A

arterial blood.

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18
Q

Most analyzed blood gasses in arterial blood are…

A

CO2, O2, and pH.

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19
Q

The blood specimen that does NOT have oxygen is…

A

venous blood.
(Contains only CO2)

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20
Q

Arterial Blood & Venous Blood:
Which one is easier to take?

A

Venous blood.

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21
Q

Venous blood specimens can be…

A

1) Whole blood.
2) Plasma.
3) Serum.

22
Q

The largest sources of biomarkers whether for diagnostics or therapeutics are…

A

Blood serum & blood plasma.

23
Q

The blood consists of two parts, which are…

A
  • The fluid part (plasma).
  • The cellular part (RBCs, WBCs, platelets).
24
Q

When we do not separate blood into its constituents, that blood is called…

25
Which part of the blood has biomarkers?
The fluid part; the plasma.
26
A blood sample put in a plain tube which is left to stand for 20-30 mins...
clots.
27
A clotted blood sample centrifuged ends up as a...
Two part tube: - Upper part (supernatant): serum. - Lower part (precipitate): NO blood clotting factors; because they have been consumed.
28
A blood sample put in a tube with an anticoagulant (e.g., heparin) and inverted (not shaken; to avoid hemolysis!) does NOT...
clot. (the anticoagulant prevents blood clotting)
29
A blood sample in a tube with anticoagulant, centrifuged, ends up as a...
1) Plasma (supernatant). 2) Buffy coat (white blood cells and platelets). 3) Red blood cells. DIFFERENCE FROM SERUM: it has clotting factors!
30
In a biochemical test, when the analyte is a clotting factor, we use...
A blood sample in a tube with anticoagulant. (SERUM DOESN"T HAVE CLOTTING FACTORS!)
31
For unstable analytes in biochemical testing, do we use the serum or plasma?
Plasma. (serum requires 20-30m standing; risk of analyte deteriorating)
32
In summary, plasma samples are used commonly when there are...
1) Unstable analytes. 2) Emergencies. 3) Clotting factor analytes.
33
The most common advantage & the most common disadvantage of urine as a specimen sample is...
- Advantage: non-invasive, easy to take. - Disadvantage: variable urine volume.
34
Enumerate urine sample types...
1) Random urine sample. 2) 24-hour urine sample. 3) Early-morning/overnight fasting urine sample. 4) Mid-stream urine sample.
35
What type of testing are RANDOM urine samples NOT appropriate for?
quantitative testing. (random urine samples are ONLY used qualitatively, i.e., is it absent? yes/no, not how much!)
36
What type of urine sample used for concentration/quantitative testing?
24-hour urine sample.
37
The most concentrated urine sample is...
Early morning overnight fasting urine sample. (PREFERRED FOR MICROSCOPIC EXAMINATIONS)
38
When we want to screen for HSG, the pregnancy hormone, the best urine sample to take is... (and why?)
Early morning overnight fasting urine sample. BECAUSE: most concentrated specimen) (PREFERRED FOR MICROSCOPIC EXAMINATIONS)
39
Mid-stream urine samples are mostly used for...
urine culture.
40
Why don't we use preservatives for mid-stream urine samples?
It would kill the biotics; rendering the sample useless.
41
A good mid-stream urine sample is...
Fresh & has no preservatives.
42
The most common preservatives used in urine samples are...
1) Freeze. 2) Glacial acetic acid. 3) Nitric acid.
43
We use preservatives (or acids) in urine samples that are NOT for urine culture purposes because...
1) To inhibit bacterial growth (preservative). 2) To stabilize certain metabolites (acids).
44
TRUE/FALSE: We can use preservatives or acids in urine samples meant for urine culture???
FALSE!
45
A specimen is dangerous when...
it carries a dangerous infection. (like hepatitis B and HIV)
46
Dangerous specimens and their request forms must be...
labelled with a yellow "dangerous specimen!" sticker.
47
Ways in which biochemical results are expressed...
1) Qualitative: presence or absence; yes/no? NOT COMMON. 2) Semi quantitative: glucose in urine (+ urine > +++ urine). NOT COMMON. 3) Quantitative (how much?) COMMON: result = concentrations (solute/solvent).
48
Patient test results are compared to...
published statistically normal/abnormal reference ranges.
49
The trend in comparing patient result results to reference ranges...???
LESS than normal? HYPO-. NORMAL? normal. MORE than normal? HYPER-.
50
TRUE/FALSE: Reference intervals are always fixed.
FALSE!!! They may vary depending on the age or sex of the patient.
51