Experiment 1A Flashcards
Test for the deoxyribose in
DNA
Dische Diphenylamine Test
Reagents for Dische Diphenylamine Test
diphenylamine, Sulfuric acid
Positive result for Dische Diphenylamine Test
light blue to blue depending on the amount of DNA
Chemical Reaction of Dische Diphenylamine Test
Diphenylamine solution – a mixture of diphenylamine, glacial acetic acid and sulfuric acid
General test for proteins, detecting peptide bonds
Biuret Test
Reagents for Biuret Test
KOH, hydrated CuSO4, Potassium sodium tartarate
Positive result for Biuret Test
Violet solution
Chemical Reaction of Biuret Test
The reaction is a formation of complex between copper and oxygen and between copper and nitrogen
General test for carbohydrates
Molisch Test
Reagents for Molisch Test
α-naphthol in ethanol, Sulfuric Acid
Positive result for Molisch Test
purple ring
Chemical Reaction of Molisch Test
The reaction is a dehydration reaction forming a furfural compound. The α-naphthol forms a complex that gives the positive sample a purple ring
General Test for Lipids
Sudan Test
is non-polar and is soluble only to lipids and similar macromolecules
Sudan IV
Reagents for Sudan Test
Sudan IV (red brown crystals)
