SECTIONING

Question 1

correct shape of the tissue block

Answer 1

4-sided prism/truncated pyramid

Question 2

recommended thickness for routine tissue microtomy

Answer 2

4-6 u

Question 3

recommended thickness for frozen sections

Answer 3

10-15 u

Question 4

recommended thickness for electron microscopy

Answer 4

0.5 u

Question 5

kinds of microtome:

simplest but difficult to reorient the block

Answer 5

rocking microtome

Question 6

kinds of microtome:

most common microtome used for paraffin embedded blocks

Answer 6

rotary/minot microtome

Question 7

kinds of microtome:

most dangerous type due to movable exposed knife

Answer 7

sliding microtome

Question 8

type of sliding microtome:

knife: stationary
block: moving
for all forms of media

Answer 8

base sledge

Question 9

type of sliding microtome:

knife: moving
block: stationary

Answer 9

standard sliding microtome

Question 10

kinds of microtome:

used for unfixed, unfrozen specimen for enzyme demonstration

Answer 10

vibrotome

Question 11

kinds of microtome:

for electron microscopy

Answer 11

ultrathin microtome

Question 12

what knives are used for electron microscopy

Answer 12

diamond knives or broken plate glass

Question 13

fixative and embedding media for ultrathin microtome

Answer 13

fixative: osmium tetroxide

embedding media: plastic

Question 14

temperature of floatation bath

Answer 14

45-50 C or 6-10 C below melting point

Question 15

color of floatation bath

Answer 15

black