Enzyme Kinetics Flashcards
1
Q
What is Km constant?
A
It is the value of substrate concentration which = 1/2 Vmax
2
Q
Why is a line weaver and burk plots used?
A
As Vmax is difficult to calculate
3
Q
How is Km calculated from a lineweaver and burk plot?
A
X intercept = -1/Km
4
Q
How is Vmax calculated from a lineweaver and burk plot?
A
Y intercept = 1/ Vmax
5
Q
What is the beer-lambert law?
A
If a solution follows the beer-lambert law we can calculate the concentration from the absorbance
6
Q
What does mM mean in terms of concentration?
A
20mM = 20 mol/l = 20mol/ml = 20 umol/uL = 20 pmol/pl
