1.1 The microscope in cell studies Flashcards
(8 cards)
1
Q
how to prepare microscope slide using liquid specimen
A
- add few drops containing sample to clean slide using pipette
- lower coverslip over specimen gently press down to remove air bubbles
2
Q
how to prepare microscope slide using solid specimen
A
- use scissors/scalpel to cut small sample of tissue, peel away/cut v thin layer of cells from tissue sample
- place sample onto slide (drop of water)
- apply iodine stain
- lower coverslip
3
Q
how to prepare slide using human cells
A
- brush teeth thoroughly
- sterile cotton swab scrape inside cheek surface 5-10s
- smear swab on centre of slide 2-3s
- drop of methylene blue
- coverslip
- absorb excess solution by allowing paper towel to touch one side of coverslip
4
Q
common stains
A
- iodine
- crystal violet
- methylene blue
- congo red
5
Q
use of iodine
A
- stains starch blue-black
- colour nuclei and plant cell walls pale yellow
6
Q
crystal violet use
A
stain cell walls purple
7
Q
use of methylene blue
A
stains animal cell nuclei dark blue
8
Q
use of congo red
A
- not taken up by cells
- stains background red (contrast with any cells present)