DNA sequencing

Question 1

DNA sequencing 1/11

Answer 1

DNA is mixed with primers, polymerase, nucleotide and terminators

Question 2

DNA sequencing 2/11

Answer 2

Placed in a thermal cycler

Question 3

DNA sequencing 3/11

Answer 3

at 96oc the DNA strands seperate

Question 4

DNA sequencing 4/11

Answer 4

at 50oc primers anneal to strand ends

Question 5

DNA sequencing 5/11

Answer 5

at 60oc DNA polyemrase starts to build new DNA strands

Question 6

DNA sequencing 6/11

Answer 6

Everytime a terminator is used instead of a nucleotide no more bases can be added

Question 7

DNA sequencing 7/11

Answer 7

This causes many bases varying in length

Question 8

DNA sequencing 8/11

Answer 8

DNA fragments are separated by capillary sequencing

Question 9

DNA sequencing 9/11

Answer 9

Florescent tags used to identify terminator bases using lasers

Question 10

DNA sequencing 10/11

Answer 10

Order of nucleotides shows sequence of new complimentary strands

Question 11

DNA sequencing 11/11

Answer 11

Computer anaylses overlaps in code in fragments