Module 7 - Microbial Genetics Flashcards
What term describes the determination and study of complete genomic sequences?
Genomics
The entire genome of an organism is called?
Genomic library
In what way have researches relied on genomic libraries for genetic analyses?
Determining sequences of the cloned fragments
The basic unit of heredity that occupies a specific location on a chromosome is called?
Gene
The entire set of genetic instructions found in a cell is called?
Genome
Which sequencing takes advantage of the primer-specific DNA synthesis activity of DNA polymerase?
Sanger or dideoxy sequencing
What are the three steps in Sanger sequencing?
- Cloning of the fragment to be sequences
- DNA synthesis
- Gel electrophoresis
How does Sanger sequencing differ from PCR?
For Sanger sequencing, only a single primer is used in the reaction compared to two
What purpose does Sanger sequencing serve in?
Research purposes, such as targeting smaller genomic regions in a larger number of samples
- Low error rate
Which sequencing method is based on the synthesis of a DNA strand, using a template strand to guide the insertions of nucleotides
Sanger Sequencing
Which method of sequencing involves first sequencing the cloned DNA as far as possible then, the newly obtained sequence information is used to design another sequencing primer
Primer walking
Which method of sequencing is similar to Sanger sequencing in that it relies on DNA polymerase-mediated synthesis of the complementary strand of a single-stranded template?
Pyrosequencing
When is a dNTP incorporated into a growing piece of DNA in pyrosequencing?
Only if the dNTP is complementary to the template base
What is released when the dNTP is incorporated into DNA, in pyrosequencing?
Pyrophosphate (PPi)
Which enzyme converts pyrophosphate into ATP in pyrosequencing?
ATP-sulfurylase
