pcr

Question 1

anneal

Answer 1

The joining of two molecules, for example two complementary DNA strands during the cooling phase of PCR.

Question 2

Forward primer

Answer 2

A technique that separates DNA fragments based on their molecular size.

Question 3

Polymerase chain reaction (PCR)

Answer 3

A laboratory technique used to produce many identical copies of DNA from a small initial sample.

Question 4

Primer

Answer 4

A short, single strand of nucleic acids that acts as a starting point for polymerase enzymes to attach.

Question 5

Reverse primer

Answer 5

A DNA primer that binds to the 3’ end of the coding strand and reads the DNA in the reverse direction to RNA polymerase.

Question 6

Taq polymerase

Answer 6

A heat-resistant DNA polymerase enzyme isolated from the bacteria Thermus aquaticus, which amplifies a single-stranded DNA molecule by attaching complementary nucleotides in PCR (at 72°C).