Lecture 15 -- Protein Transport and Secretion II Flashcards
1
Q
- uses sec or tat first
- found in plants and animals, involved in adaptation, virulence, and pathogenicity
- proteins folded before being transported
- undergoes assembly when needed – pseudopilus extends
A
Type II Secretion System
2
Q
- outer-membrane complex (gated channel)
- pseudopilus (aids in the movement of the substrate through the channel)
- inner membrane platform (targets substrate, involved in protein folding and contains ATPase which provides energy for the system)
A
Type II Secretion System Structure
3
Q
- proteases, cyto/hemolysis, adhesins, growth inhibition of competing bacteria, intracellular motility
- uses sec
- portion of protein facilitates its own secretion
- energy-independent
- remains unfolded
- beta barrel
A
Type V Secretion System
4
Q
- signal peptide
- pertactin domain that aids in folding and transport
- autotransporter domain
A
autotransporter structure
5
Q
- c terminal signal sequence uses ABC transporter for energu, PMF has elecrophoresis early exit –> uses proton racheting to prevent backliding
- toxins
- cannot secrete proteins inside another cell
A
Type I secretion
6
Q
- secretes proteins and DNA proteins
- proteins have translocation signal used for targeting
- pilus is also a channel
- proteins remain unfolded
A
Type IV
7
Q
- substrates effector proteins
- injectosomes (needle)
- cell adherence is the trigger/requires ATP and PMF
- substrates unfolded and LACK SIGNAL SEQUENCE
- are assembled when needed
A
Type III
8
Q
- effectors include function for competing with other bacteria
- major role in microbiome
- contain immunity proteins (inhibits action of toxin/reverses action of effector) and phsyical barriers
- has tip containing effector (drives up and punches through adjacent cell)
A
Type VI
