Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Microbiology > 2.4 > Flashcards

2.4 Flashcards

1
Q

b cell receptor:

forms of antigens recognized

A

macromolecules
small chemicals
conformational and linear epitopes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

b cell receptor:

diversity

A

each clone has a unique specificity; potential for >10^9 distinct specificities

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

b cell receptor:

antigen recognition is mediated by:

A

variable regions of heavy and light chains of membrane Ig

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

b cell receptor:

signaling functions are mediated by:

A

proteins associated with membrane Ig

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

b cell receptor:

effector functions are mediated by:

A

constant regions of secreted Ig

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

t cell receptor:

forms of antigens recognized

A

mainly peptides displayed by mhc molecules on APcs

linear epitopes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

t cell receptor:

diversity

A

each clone has a unique specificity; potential for >10^11 distinct specificities

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

t cell receptor:

antigen recognition is mediated by:

A

variable regions of alpha and beta chains of the TCR

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

t cell receptor:

signaling functions are mediated by:

A

proteins associated with the TCR

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

t cell receptor:

effector functions are mediated by:

A

TCR does not perform effector functions

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

CDRs =

A

3 variable
antigen-binding
loops

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q 
isotype of antibody: IgA
subtypes (H chain):
serum concentration (mg/mL):
serum half life (days):
secreted form:
functions:
A 
IGA 1, 2
3.5
6
mainly dimer, also monomer, trimer 
mucosal immunity
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q 
isotype of antibody: IgD
subtypes (H chain):
serum concentration (mg/mL):
serum half life (days):
secreted form:
functions:
A 
none 
trace
3 
monomer 
naive b cell antigen receptor
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q 
isotype of antibody: IgE
subtypes (H chain):
serum concentration (mg/mL):
serum half life (days):
secreted form:
functions:
A 
none
0.05 
2
monomer 
defense against helminthic parasites, immediate hypersensititivty
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q 
isotype of antibody: IgG
subtypes (H chain):
serum concentration (mg/mL):
serum half life (days):
secreted form:
functions:
A 
IgG 1-4 (gamma 1, 2, 3, 4)
13.5
23
monomer 
opsonization, complement activation, ab dependent cell mediated cytotoxicity, neonatal immunity, feedback inhibition of B cells
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q 
isotype of antibody: IgM
subtypes (H chain):
serum concentration (mg/mL):
serum half life (days):
secreted form:
functions:
A 
none
1.5
5
pentamer
naive b cell antigen receptor (monomeric form), complement activation
17
Q

Generation of hybridomas and monoclonal antibodies

A

isolate spleen cells from mouse immunized with antigen C
fusion
fuse spleen cells and immortal myeloma cell line
culture in selection medium
only fused cells (hybridomas) grow
isolate clones derived from single hybridoma cells
screen supernatants and expand positive clones
hybridomas producing monoclonal anti-x antibody

18
Q

featureL
immunoglobulin (Ig)
T cell receptor (TCR)

A
19
Q

feature: antigen binding
immunoglobulin (Ig)
T cell receptor (TCR)

A

made up of three crds in Vh and three crds in Vl

made up of three cdrs in Va and three cdrs in Vb

20
Q

feature: changes in constant regions
immunoglobulin (Ig)
T cell receptor (TCR)

A

heavy chain class switching and change from membrane to secretory Ig

none

21
Q

feature: affinity of antigen binding
immunoglobulin (Ig)
T cell receptor (TCR)

A

Kd 10^7-10^11 M; average affinity of Igs increases during immune responses to protein antigens

Kd 10^5-10^7 M; no change during immune responses

22
Q

feature: off rate and on rate
immunoglobulin (Ig)
T cell receptor (TCR)

A

rapid on rate, variable off rate

slow on rate, slow off rate

23
Q

Steps in maturation of lymphocytes (6)

A 
communitment 
prolifertion
pre-B/T antigen receptor expression
proliferation
antigen receptor expression 
positive and negative selection
24
Q

Steps in the maturation and selection of MHC-restricted T lymphocytes (4)

A 
stem cell
double negative pro t cell 
pre t cell 
double positive immature t cell 
mature cd4/cd8/apoptosis
25
Q

weak recognition of class 2 mhc + peptide

A

-positive selection

26
Q

weak recognition of class 1 mhc + peptide

A

-positive selection

27
Q

no recognition of mhc + peptide

A

-failure of positive selection (death by neglect)

28
Q

strong recognition of either class 1 or class 2 mhc + peptide

A

-negative selection

Microbiology (42 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions