Serological testing Monospot test.

Question 1

____________is used to detect infectious mononucleosis which is a self-limiting
disease caused by the Epstein-Barr virus.

Answer 1

Monospot test

Question 2

mononucleosis It is typically seen in

Answer 2

young adults and children less than 5 years of age

Question 3

What issue
Clinically, this disease presents as fever, malaise, lethargy, sore throat with exudates, enlarged lymph nodes on the neck, mild hepatitis, enlarged spleen, and occasional blotchy skin rash.

Answer 3

infectious mononucleosis

Question 4

Infectious mononucleosis causes the accumulation of increased numbers and abnormal forms of lymphocytes and monocytes in the lymph nodes, thus causing formation of _______ antibody.

Answer 4

“heterophile”

Question 5

________ is an antibody that is structurally similar to the antibody that is specifically produced as a response to antigen stimulation

Answer 5

heterophile antibody

Question 6

True/false
Monospot test
Collect venous blood in tubes with or without anticoagulant

Answer 6

True

Question 7

Prior to Testing Monospot test

Test the specimen within ______ of blood collection if stored between ____ degrees Celsius.

Answer 7

24 hours

2-8 degrees Celsius

Question 8

True/False

Do not use hemolyzed or contaminated samples

Answer 8

True

Question 9

Testing Procedure

Allow the Latex Reagent and controls to reach room temperature at what range?

Answer 9

20 to 30 degrees C

Question 10

Testing Procedure
Gently shake the Latex Reagent vial to disperse and suspend the latex particles in the buffer solution. Vigorous shaking should be _______

Answer 10

avoided

Question 11

True/False
Testing Procedure
Use the dispenser to transfer the specimen to the card, dispense 3 drops

Answer 11

FALSE

1 drop

Question 12

Testing Procedure

Add ____ drop(s) of Latex Reagent next to the drop of sample

Answer 12

one

Question 13

Testing Procedure

Gently rotate the slide for ________ manually or on a rotatory shaker set at _____ rpm.

Answer 13

3 minutes

60-100 RPM

Question 14

Testing Procedure

Look for the presence or absence of ______ after rotating

Answer 14

agglutination

Question 15

Testing Procedure

Report results as either

Answer 15

positive or negative

Question 16

POS/NEG
Monospot Test Results
-Shows agglutination.

Answer 16

Positive result

Question 17

POS/NEG
Monospot Test Results
-Demonstrates a smooth, homogenous solution. No Agglutination

Answer 17

Negative result

Question 18

What ensures the accuracy and reliability of test results

Answer 18

Quality control

Question 19

Quality control Divided into two sections includes….

Answer 19

QC equipment: Calibrate to 100 rpm

CQ Reagents: positive and negative controls

Question 20

True/FAlse

Control reagents are treated as patients and run every time an unknown sample is tested.

Answer 20

True