Gram staining technique and interpretation

Question 1

What is gram staining?

Answer 1

Gram staining is a technique used to determine whether a bacteria is gram positive or negative(Determine structure of cell wall)

Question 2

Step 1 of gram staining:

Answer 2

Application of crystal violet (purple dye)

Question 3

Step 2 of gram staining:

Answer 3

Application of iodine (mordant that affixes dye to cells)

Question 4

Step 3 of gram staining:

Answer 4

Alcohol wash (decolorizes)

Question 5

Step 4 of gram staining:

Answer 5

Application of safarnin (counterstain, dyes gram negative bacteria

Question 6

Gram positive bacteria stain _____

Answer 6

Purple

Question 7

Gram negative bacteria stain _____

Answer 7

red/pink