Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

IUMICRO > Biochemicals > Flashcards

Biochemicals Flashcards

1
Q

METHYL RED (MR) TEST

  1. Purpose/Principle:
  2. Substrate:
  3. Product(s):
  4. Reagent:
  5. Enzymes:
  6. Pathway(s):
  7. Results:
A
  1. An organism’s ability to produce acid end products from glucose fermentation
  2. Glucose
  3. Mixed acids
  4. Methyl Red Indicator
  5. NONE
  6. Mixed Acid fermentation
  7. Positive: Red
    Negative: Yellow (no color change)
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

VOGES-PROSKAUER (VP) TEST

  1. Purpose/Principle:
  2. Substrate:
  3. Product(s):
  4. Reagent:
  5. Enzymes:
  6. Pathway(s):
  7. Results:
A
  1. To detect acetoin, a neutral end product, from glucose fermentation
  2. Glucose
  3. Acetoin
  4. KOH and Alpha naphthol
  5. NONE
  6. Butylene Glycol fermentation
  7. Positive: Red/pink (upper layer of media)
    Negative: Yellow (no color change)
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

SODIUM CITRATE TEST

  1. Purpose/Principle:
  2. Substrate:
  3. Product(s):
  4. Reagent:
  5. Enzymes:
  6. Pathway(s):
  7. Results:
A
  1. An organism’s ability to use citrate as its sole source of carbon
  2. Citrate
  3. NONE
  4. NONE
  5. NONE
  6. NONE
  7. Positive: Blue
    Negative: Green (no color change)
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

PHENYLALANINE DEAMINASE

  1. Purpose/Principle:
  2. Substrate:
  3. Product(s):
  4. Reagent:
  5. Enzymes:
  6. Pathway(s):
  7. Results:
A
  1. An organism’s ability to oxidatively deaminate phenylalanine to phenylpyruvic acid (remove an amine group from an amino acid)
  2. Phenylalanine
  3. Phenylpyruvic acid
  4. Ferric chloride (FeCl3)
  5. Phenylalanine deaminase
  6. NONE
  7. Positive: Dark green slant (fades after a few minutes)
    Negative: Yellow (no color change - from reagent)
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

INDOLE TEST - EHRLICH’S PROCEDURE

  1. Purpose/Principle:
  2. Substrate:
  3. Product(s):
  4. Reagent:
  5. Enzymes:
  6. Pathway(s):
  7. Results:
  • Differences from Kovac’s Procedure??
    ** Used specifically for??
    *** Used specifically for (Kovac’s Procedure)??
A
  1. An organism’s ability to split indole from tryptophan
  2. Tryptophan
  3. Indole
  4. Kovac’s (dimethylaminobenzaldehyde)
  5. Tryptophanase
  6. NONE
  7. Positive: Red (middle layer in Ehrlich’s’ top layer in Kovac’s)
    Negative: Yellow (no color change)

Extraction of indole with xylene, chloroform, or ether prior to
reagent
** Nonfermenting GNRs and anaerobes
** Enterobacteriaceae

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

MOTILITY

  1. Purpose/Principle:
  2. Substrate:
  3. Product(s):
  4. Reagent:
  5. Enzymes:
  6. Pathway(s):
  7. Results:
A
  1. To determine if an organism is motile (flagellated) or non motile (nonflagellated)
  2. None
  3. None
  4. None
  5. None
  6. None
  7. Positive: Hazy appearance/stab line not evident/red color diffused (tetrazolium salts added)
    Negative: No hazy appearance/stab line clearly seen/red color localized to stab line (tetrazolium salts added)
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

UREASE TEST

  1. Purpose/Principle:
  2. Substrate:
  3. Product(s):
  4. Reagent:
  5. Enzymes:
  6. Pathway(s):
  7. Results:

*Media name??

A
  1. An organism’s ability to split urea by urease
  2. Urea
  3. Ammonia
  4. None
  5. Urease
  6. None
  7. Positive: Pink
    Negative: Yellow (no color change)
  • Christensen’s
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

DECARBOXYLASE MEDIA (LYSINE)

  1. Purpose/Principle:
  2. Substrate:
  3. Product(s):
  4. Reagent:
  5. Enzymes:
  6. Pathway(s):
  7. Results:

*Media name??

A
  1. Purpose/Principle: To detect the presence of decarboxylase enzymes in the breakdown of amino acids resulting in amine + CO2
  2. Substrate: Lysine
  3. Product(s): Cadaverine + CO2
  4. Reagent: NONE
  5. Enzymes: Lysine decarboxylase
  6. Pathway(s): NONE
  7. Results: Positive: Purple/Brown
    Negative: Yellow
  • Moeller’s
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

DECARBOXYLASE MEDIA (ORNITHINE)

  1. Purpose/Principle:
  2. Substrate:
  3. Product(s):
  4. Reagent:
  5. Enzymes:
  6. Pathway(s):
  7. Results:

*Media name??

A
  1. Purpose/Principle: To detect the presence of decarboxylase enzymes in the breakdown of amino acids resulting in amine + CO2
  2. Substrate: Ornithine
  3. Product(s): Putrescine + CO2
  4. Reagent: NONE
  5. Enzymes: Ornithine decarboxylase
  6. Pathway(s): NONE
  7. Results: Positive: Purple/Brown
    Negative: Yellow
  • Moeller’s
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

ARGININE DIHYDROLASE

  1. Purpose/Principle:
  2. Substrate:
  3. Product(s):
  4. Reagent:
  5. Enzymes:
  6. Pathway(s):
  7. Results:

*Media name??

A
  1. Purpose/Principle: To detect the presence of arginine dihydrolase enzymes in the breakdown of the amino acid, arginine
  2. Substrate: Arginine
  3. Product(s): Ornithine
  4. Reagent: NONE
  5. Enzymes: Arginine Dehydrolase
  6. Pathway(s): NONE
  7. Results: Positive: Purple/Brown
    Negative: Yellow
  • Moeller’s
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

KLIGLER IRON AGAR (KIA)

  1. Purpose/Principle:
  2. Substrate:
  3. Product(s):
  4. Reagent:
  5. Enzymes:
  6. Pathway(s):
  7. Results:

*Triple Sugar Iron Agar (TSI) difference??

A
  1. An organism’s ability to ferment lactose and/or glucose, with/without the production of gas – the production of hydrogen sulfide is also determined
  2. Glucose, Lactose, Sodium thiosulfate
  3. Acid/Basic products, H2S (organism-dependent)
  4. NONE
  5. NONE
  6. NONE
  7. A/A = glucose and lactose fermentation
    K/A = glucose only/no lactose fermentation
    K/K = no glucose fermentation
    Bubbles = CO2 gas production
    Black ppt. = H2S production

*Contains 1.0% sucrose, as well as glucose (0.1%) and lactose (1.0%) – when yellow on the slant, could be from lactose or sucrose utilization

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

DECARBOXYLASE BASE

Purpose?
Results?

A

To ensure organism is producing acid by glucose fermentation – used as a control tube for the LOA

Yellow color required for valid interpretation of LOA series

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

1% CARBOHYDRATES

  1. Purpose/Principle:
  2. Substrate:
  3. Product(s):
  4. Reagent:
  5. Enzymes:
  6. Pathway(s):
  7. Results:
A
  1. An organism’s ability to degrade specific carbs with the production of acids, with/without visible gas
  2. Various carbohydrates
  3. Acid products (organism-dependent)
  4. None
  5. None
  6. None
  7. Positive: Yellow
    Negative: Purple (no color change)
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

OXIDATION/FERMENTATION (OF) MEDIA

  1. Purpose/Principle:
  2. Substrate:
  3. Product(s):
  4. Reagent:
  5. Enzymes:
  6. Pathway(s):
  7. Results:
A
  1. To determine an organism’s mechanism for utilization of glucose; fermentative, oxidative or neither
  2. Glucose (or other carbs)
  3. Acid products (organism-dependent)
  4. None
  5. None
  6. None
  7. Positive: Yellow in oiled tube (fermenter)
    Yellow in unoiled tube (oxidizer)
    Negative: Green (no color change) – asaccharolytic (non-
    oxidizer)
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

CYTOCHROME OXIDASE

  1. Purpose/Principle:
  2. Substrate:
  3. Product(s):
  4. Reagent:
  5. Enzymes:
  6. Pathway(s):
  7. Results:
A
  1. To detect the presence of oxidized cytochrome C
  3. 4.1% tetramethyl-p-phenylenediamine dihydrochloride or
    Dimethyl-p-phenylendiamine + alpha napthol
  4. Cytochrome oxidase
  5. None
  6. Positive: Blue
    Negative: No color change
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

NITRATE TEST

  1. Purpose/Principle:
  2. Substrate:
  3. Product(s):
  4. Reagent:
  5. Enzymes:
  6. Pathway(s):
  7. Results:
A
  1. An organism’s ability to reduce nitrate to nitrite or nitrate to
    nitrogen gas
  2. Nitrate
  3. Nitrite or Nitrogen gas
  4. Alpha naphthylamine, sulfonilic acid, zinc dust
  5. Nitrate reductase
  6. None
  7. Positive: Red after addition of two reagents or clear after
    addition of two reagents and addition of Zinc dust
    Negative: Clear after addition of two reagents and Red after
    addition of Zinc dust
17
Q

MUG TEST

  1. Purpose/Principle:
  2. Substrate:
  3. Product(s):
  4. Reagent:
  5. Enzymes:
  6. Pathway(s):
  7. Results:
A
  1. An organism’s ability to produce beta-glucuronidase
  2. None
  3. None
  4. None
  5. beta-glucuronidase
  6. None
  7. Positive: fluorescence with UV light
    Negative: no fluorescence

IUMICRO (25 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions