120114 hematopoiesis

Question 1

where is blood formed in the embryo?

Answer 1

yolk sac, then liver, then bone marrow (5th month)

Question 2

red marrow

Answer 2

hematopoietic (making blood)

Question 3

after age 20, marrow becomes yellow where?

Answer 3

long bones

Question 4

where does red marrow persist throughout life?

Answer 4

flat bones

Question 5

all marrow cells come from

Answer 5

multipotent master stem cell

Question 6

HSCs go on to form

Answer 6

myeloid and lymphoid cells

Question 7

hemangioblasts go on to form

Answer 7

endothelial cells

HSCs (hematopoietic stem cells)

Question 8

multipotent master stem cell goes on to form

Answer 8

hemangioblasts and MSCs

Question 9

what markers are on HSCs?

Answer 9

CD117 (c kit +)
CD34
Lin -

Question 10

what cells are CD45 - ?

Answer 10

megakaryocyte

erythroid

Question 11

CDs are used in what technique?

Answer 11

fluorescent activated cell sorting

Question 12

myeloid means

Answer 12

all leukocytes except lymphocytes

includes granulocytes, monocytes, megakaryocytes (platelets)

Question 13

colony forming units

Answer 13

each cell type arises from a colony forming unit

Question 14

colony forming units are induced by

Answer 14

growth factors or cytokines called CSFs (colony stimulating factors)

Question 15

multipotent CSF ex

Answer 15

stem cell factor is a multipotent CSF that binds c-Kit to form all myeloid and lymphoid CFUs

Question 16

unipotent CSF ex

Answer 16

erythropoietin (works on CFU-E for erythropoiesis)
thrombopoietin
G-CSF
M-CSF

Question 17

morphology of erythroid and granulocyte lineages in general

Answer 17

cell size gets smaller
nuclear morphology can tell you the stage

cytoplasm from blue to red (blue is RNA, red is protein) for erythroids

specific granules

Question 18

stages of differentiation from proerythroblast

Answer 18

basophilic erythroblast
polychromatic erythroblast
orthochromatic erythroblast (normoblast)
reticulocyte
erythrocyte

Question 19

stages from myelobast

Answer 19

promyelocyte
mylocyte
metamyelocyte
N band (or B, or E band)
neutrophil (or basophil, eosinophil)

Question 20

which has cytoplasm that is much less blue staining? proerythroblast or myeloblast?

Answer 20

myeoblast

Question 21

at what stage is the nucleus indented for the myeloblast lineage?

Answer 21

metamyelocyte

Question 22

at what stage is the nucleus very amorphic for the myeloblast lineage?

Answer 22

N band

Question 23

nucleus of granulocytic cells is

Answer 23

segmented (especially neutrophils)

Question 24

slide of different granulocytic and erythroid lineages

Answer 24

see slide at very end