Micro ID Tests and Their Reagents

Question 1

Reagent used in coagulase testing?

Answer 1

Plasma

Question 2

Reagent used in catalase testing?

Answer 2

3% Hydrogen peroxide (H2O2)

Question 3

Reagent used in L-pyrrolidonyl-β-naphthylamide hydrolysis? (PYR)

Answer 3

N,N-dimethylaminocinnamaldehyde

Question 4

Reagent used in modified oxidase test?

Answer 4

tetramethyl-p-phenylenediamine & Dimethyl sulfoxide (DMSO)

Question 5

Reagent used in A disk test?

Answer 5

Bacitracin

Question 6

Reagent used in SXT disk test?

Answer 6

Trimethoprim-sulfamethoxazole

Question 7

What is used in the CAMP test?

Answer 7

S. aureus (for beta-lysin toxin)
Group B strep (for CAMP factor)

Question 8

Reagent used for hippurate hydrolysis when the result is to try and get a precipitate to form?

Answer 8

7% ferric chloride

Question 9

Reagent used for hippurate hydrolysis when the wanted result is a blue/purple color change?

Answer 9

Ninhydrin

Question 10

Reagent used in P disk susceptibility?

Answer 10

Optochin (ethylhydrocupreine hydrochloride)

Question 11

Reagent used in bile solubility?

Answer 11

Bile salts (sodium deoxycholate & sodium taurocholate)