Lecture 9: Virus Engineering Flashcards
How can virus recombinants be selected by plaquing on selective medium?
Define homologous recombination. How can we select for viruses after homologous recombination?
What is spontaneous genome packing in vitro?
Define transfection. How can virus genomes be transfected into the cell?
How can a recombinant virus be engineered?
How was the poxvirus genome reconstructed entirely from chemically synthesized DNA?
Describe genetic complementation.
How is genetic complementation used to make a modified genome?
How can a virus capsid be used to deliver a therapeutic gene into a human cell?
Define pseudotyping.What is a good example of pseudotyping?
While inserting influenza viral genomes into plasmids, why do there need to be two plasmids for all 8 segments?
What are some critical genes found on plasmid 1 vs plasmid 2 during the process of creating a flu vaccine?
