Transformation methods for eukaryotes

Question 1

What’s the mechanism behind T-DNA transfer?

Answer 1

1. Plant is recognized if wounded by virA protein in bacteria.
2. Ti-plasmid is produced (with left- and righ border).
3. The bacteria transfers all its genetic material from within the left- and right borders into the plant cell.

Question 2

What’s a floral dip?

Answer 2

A floral dip is when you dip an entire plant (including seeds) in an agrobacterium suspension. The seeds become transgenic with whatever genetic material you introduce through T-DNA transfer.