Translplants - b cell

Question 1

3 phases of antibody mediated rejection

Answer 1

Phase 1 - B cells recognise

Phse 3 -this is in the ENDOTHELIUM whereas in t-cell mediated, the t-cells go through the interstitium and cause tubular disease

Question 2

What ways do antibodies use to cause injury?

Answer 2

Complement activated - cause holes in the endothelium, can also recruit other cells, and release cytokines

Complement independent

you essentially get loads of cells in the endothelium of the kidney, causing damage

This causes glomerulitis

Question 3

Which type of rejection is harder to treat?

Answer 3

T cell mediated injury gets better, and the creatinine comes down

Antibody mediated rejection - harder to treat

Question 4

Are anti-HLA antibodies naturally occuring? When do they occur?

Answer 4

No - they are either pre-formed during pregnancy, transfusion

Or they are formed after transplantation

Question 5

What are the three types of essays used to test for anti-HLA antibodies after transplantation?

Answer 5

Cytotoxicity assay
Flow cytometry
Solid phase assays (using synthetic beads coated with different HLA

Answer 6

Plasma exchange

Question 7

Viral infections after

Answer 7

BK nephropathy

Question 8

Acute t-cell mediated rejection treatment

Answer 8

Steroids - prednisolone

Question 9

If the recipient has a detectable antibody in the serum against a HLA type, is this high risk or low risk transplantation?

Answer 9

High risk as they already have